EB病毒IgM（EBvIgM）ELISA檢測(cè)試劑盒檢測(cè)的原理：試劑盒采用雙抗體一步夾心法酶聯(lián)免疫吸附試驗(yàn)（ELISA）。 報(bào)價(jià)，檢測(cè)原理，使用說(shuō)明書。廠家現(xiàn)貨直銷，齊一生物科技專業(yè)生產(chǎn)研發(fā)經(jīng)營(yíng)穩(wěn)定、優(yōu)質(zhì)、高效、實(shí)用的ELISA試劑盒，公司提供ELISA試劑盒免費(fèi)代測(cè)，ELISA試劑盒批發(fā)價(jià)零售，ELISA試劑盒專業(yè)生產(chǎn)廠家，ELISA試劑盒進(jìn)口品牌！全國(guó)：
試劑盒名稱：EB病毒IgM（EBvIgM）ELISA檢測(cè)試劑盒
規(guī) 格：48T/96T
品牌：進(jìn)口原裝/分裝/國(guó)產(chǎn)
產(chǎn)品的用途：僅供科研究使用！
價(jià)格：電議,咨詢?cè)诰€客服,到我司qysw@qiyibio.。齊一生物科技（上海）有限公司提供大量品牌進(jìn)口原裝、以及穩(wěn)定性強(qiáng)的國(guó)產(chǎn)ELISA試劑盒，性價(jià)比*歡迎您前來(lái)選購(gòu)！
操作步驟
實(shí)驗(yàn)開(kāi)始前，請(qǐng)?zhí)崆芭渲煤盟性噭?，試劑或樣品稀釋時(shí)，均需混勻，混勻時(shí)盡量避免起泡。每次檢測(cè)都應(yīng)該做標(biāo)準(zhǔn)曲線。如樣品濃度過(guò)高時(shí)，用樣品稀釋液進(jìn)行稀釋，以使樣品符合試劑盒的檢測(cè)范圍。
1. 加樣：分別設(shè)空白孔、標(biāo)準(zhǔn)孔、待測(cè)樣品孔?？瞻卓准訕悠废♂屢?00μl，余孔分別加標(biāo)準(zhǔn)品或待測(cè)樣品100μl，注意不要有氣泡，加樣將樣品加于酶標(biāo)板孔底部，盡量不觸及孔壁，輕輕晃動(dòng)混勻，酶標(biāo)板加上蓋或覆膜，37℃反應(yīng)120分鐘。
為保證實(shí)驗(yàn)結(jié)果有效性，每次實(shí)驗(yàn)請(qǐng)使用新的標(biāo)準(zhǔn)品溶液。
2. 棄去液體，甩干，不用洗滌。每孔加生物素標(biāo)記抗體工作液 100μl（取1μl生物素標(biāo)記抗體加99μl生物素標(biāo)記抗體稀釋液的比例配制，輕輕混勻，在使用前一小時(shí)內(nèi)配制），37℃,60分鐘。
3. 溫育60分鐘后，棄去孔內(nèi)液體，甩干，洗板3次，每次浸泡1-2分鐘，350μl/每孔，甩干。
4. 每孔加辣根過(guò)氧化物酶標(biāo)記親和素工作液（同生物素標(biāo)記抗體工作液） 100μl，37℃，60分鐘。
5. 溫育60分鐘后，棄去孔內(nèi)液體，甩干，洗板5次，每次浸泡1-2分鐘，350μl/每孔，甩干。
6. 依序每孔加底物溶液90μl，37℃避光顯色（30分鐘內(nèi)，此時(shí)肉眼可見(jiàn)標(biāo)準(zhǔn)品的前3-4孔有明顯的梯度藍(lán)色，后3-4孔梯度不明顯，即可終止）。
7. 依序每孔加終止溶液50μl，終止反應(yīng)（此時(shí)藍(lán)色立轉(zhuǎn)黃色）。終止液的加入順序應(yīng)盡量與底物液的加入順序相同。為了保證實(shí)驗(yàn)結(jié)果的準(zhǔn)確性，底物反應(yīng)時(shí)間到后應(yīng)盡快加入終止液。
8. 用酶聯(lián)儀在450nm波長(zhǎng)依序測(cè)量各孔的光密度（OD值）。 在加終止液后15分鐘以內(nèi)進(jìn)行檢測(cè)。
614035 careHPV Test, SFDA (96-test)
282605 virotype Influenza A RT-PCR Kit (96)
272703 pigtype PRRSV Ab (5)
273003 pigtype Salmonella Ab (5)
280377 virotype BVDV RT-PCR Kit (480)
9018462 Marker Kit, QC tool, QX
800372 Shipping, Postage, Packaging (express)
800373 Data Carrier
9019521 ZLV ADAPTER KIT M&N 8 STRIP
9019595 ZLV Bush, tip stripper, Cx8
9019935 Cable harness, X-axis 8 core, CQX
9020596 Tape Foam,045X750 Double Stick,DML 3000
9017843 Tool, cable, munication, RS232, QIAC
9013401 Cable set, cooling system, 90/120
9234873 ZLVPipetting Station for 2 microtit.
9013395 Tip disposal bags
9013391 Tubing set, PVC, BR9604
9013268 Cable, card-X to solenoid valve, 24V
9013222 Holder, tube, STS, single, 11 ml (30), 0
9013204 Transportation kit, BR8000
9011759 Reagent holder, 4-tube 13ml Holder for acmodating 4 microcentrifuge tubes (13 ml) on the worktable; must be used with the reagent holder tray
9011672 Reader Abdeckung spez. GSG (9194)
132605 NeXtal Stock Sodium ac. H2O (50) 1.6M Sodium acetate trihydrate - 50mL (4 x 12,5 ml tube)
800141 Investigation grade sequencing, w/ prep "Single-stranded sequencing of an unknown sequence
QIAGEN DNA template preparation
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Data assembly and sequence alignment
Accuracy >99%"
767134 PAXgene Tissue DNA Kit (50)
929605 QX Mineral Oil (50 ml)
330513 RT2 SYBR Green Fluor qPCR Mastermix (24)
330502 RT2 SYBR Green qPCR Mastermix (12)
330401 RT2 First Strand Kit (12)
9010813 BGR Stirrer PK80
69506 DNeasy Blood & Tissue Kit (250) 250 DNeasy Spin Columns, QIAGEN Proteinase K, Buffers, Collection Tubes (2 ml)
63204 DyeEx 2.0 Spin Kit (50) 50 DyeEx Spin Columns, Collection Tubes (2 ml)
211392 Internal Control DNA (High conc.)
9012755 Set, Tubing, Washer, P3
9010126 Washer boat
979015 MOTT 16S Primer 1 for Sequencing
979010 PyroMark Vacuum Prep Filter Probe (100) Re-usable filter probes for PyroMark Vacuum Workstation Q96 and Q24.
59720 EpiTect Fast 96 DNA Bisulfite Kit
9002245 QIAcube(220-240v) Promo, PrioPLUS QIAcube (220-240V) Promo Priority includes the QIAcube instrument +promo iPad, installation and extended warranty. Installation covers hardware and software installation of system and provides user with introductory training. Priority package extends warranty period of the system to 3 years. One preventive maintenance visit is included for each year of the warranty; 3 preventive maintenance visits in total. Service package includes all parts, labor and travel costs with a service response time of 48 hours (2 working days). Performance of the installation and service visits are documented on a GMP/GLP-pliant Field Service documents.
9002198 RGQ 5plex HRM, PC, PrioPLUS, no PM "RGQ 5plex, HRM, PC Instrument
Installation of RGQ 5plex HRM & Introductory training
Warranty extension to 3 yrs
Repair Center service, response 7-10 days
Loaner available in 2 days*
No preventive Maintenance
Priority service provision over non-contract customers
Documented installation & repair report
Unlimited number of repair service
All cost covered (Shipping, labor, parts)"
9002197 RGQ 5plex, PC, PrioPLUS, basic, no PM "RGQ 5plex, PC Instrument
Installation of RGQ 2plex & Introductory training
Warranty extension to 3 yrs
Repair Center service, response 7-10 days
No Loaner available
No Preventive Maintenance
Priority service provision over non-contract customers
Documented installation & repair report
Unlimited number of repair service
All cost covered (Shipping, labor, parts)"
9012718 Pipetting Position (raised platform)
9002042 Rotor-Gene Q MDx 6plex Platform
9002038 Rotor-Gene Q MDx 5plex HRM, Priority Rotor-Gene Q MDx 5plex HRM, Priority includes the Rotor-Gene Q system, installation and extended warranty. Installation covers hardware and software installation of system and provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor and travel costs with installation performance and service visits documented on a GMP/GLP-pliant Field Service documents.
59110 EpiTect 96-well Bisulfite Kit (2) 2x EpiTect Bisulfite 96-well Plates, Reaction Mix, DNA Protect Buffer, Carrier RNA, Buffers
133225 NeXtalStock Sodium ac. pH 4.8 (200)
301305 SuperFect Transfection Reagent (1.2 ml) For 40 transfections in 60 mm dishes or 160 transfections in 12-well plates
970802 PyroMark Gold Q24 Reagents (5 x 24) For performing pyrosequncing reaction on the Q24
970470 BRAF Pyro Kit (24)
970061 MGMT Pyro Kit (48)
133209 NeXtalStock MES pH 5.0 (100)
292065 mericon Sheep Kit (96)
292045 mericon Turkey (96)
929501 QX Pro Calibration Marker (0.6 ml)
9012658 Cable, X-BCR board to buffer track reade
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人癌胚抗原(CEA/CD66)ELISA試劑盒
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人基質(zhì)金屬蛋白酶抑制因子3(TIMP-3)ELISA試劑盒
人基質(zhì)金屬蛋白酶抑制因子2(TIMP-2)ELISA試劑盒
人抑瘤素M(OSM)ELISA試劑盒
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人肺表面活性物質(zhì)相關(guān)蛋白B(SP-B)ELISA試劑盒
人表面活性蛋白A(SP-A)ELISA試劑盒
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人Ⅰ型膠原C端肽(CTX-Ⅰ)ELISA試劑盒
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人促胃液素受體(GsaR)ELISA試劑盒
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