Qiagen 52304 新鮮全血樣本胞內(nèi)RNA的分離純化
操作步驟:應(yīng)用QIAGEN Plasmid Kits純化過程中,通過離心取出細(xì)菌裂解液。然后將澄清的裂解液裝入陰離子交換柱,在適當(dāng)?shù)牡望}濃度和pH值條件下,陰離子交換柱會(huì)選擇性地結(jié)合質(zhì)粒DNA。RNA、蛋白質(zhì)、代謝物和其他小分子雜質(zhì)通過中鹽濃度洗滌去除,應(yīng)用高鹽濃度緩沖夜洗脫超純的質(zhì)粒DNA(見流程圖"Comparison of QIAGEN Plasmid Kit Procedures")。通過異丙醇沉淀濃縮并脫鹽DNA,離心法收集DNA。
應(yīng)用
包含離心柱、緩沖液和收集管,用于純化100 bp以上的PCR DNA PIAN段。 通過快速、方便的結(jié)合-洗滌-洗脫步驟純化10 kb以內(nèi)的DNA PIAN段,洗脫體積為30-50 μl。內(nèi)置的pH指示劑可簡單確定DNA結(jié)合到離心柱的*pH值,整個(gè)過程可在 QIAcube上自動(dòng)化。
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Qiagen 12462 | QIAGEN Large-Construct Kit (10) | DNA質(zhì)粒純化 |
Qiagen 20021 | QIAEX II Gel Extraction Kit (150) | 膠回收純化DNA |
Qiagen 27104 | QIAprep Spin Miniprep Kit (50) | 提取高純度質(zhì)粒DNA |
Qiagen 27106 | QIAprep Spin Miniprep Kit (250) | 提取高純度質(zhì)粒DNA |
Qiagen 28004 | MinElute PCR Purification Kit (50) | 微量PCR回收試劑盒 |
Qiagen 28104 | QIAquick PCR Purification Kit (50) | PCR回收試劑盒 |
Qiagen 28106 | QIAquick PCR Purification Kit (250) | PCR回收試劑盒 |
Qiagen 28306 | QIAquick Nucleotide Removal Kit (250) | 從酶促反應(yīng)物中回收純化 |
Qiagen 28304 | QIAquick Nucleotide Removal Kit (50) | 從酶促反應(yīng)物中回收純化 |
Qiagen 28704 | QIAquick Gel Extraction Kit (50) | 膠回收試劑盒 |
Qiagen 28706 | QIAquick Gel Extraction Kit (250) | 膠回收試劑盒 |
Qiagen 30210 | Ni-NTA Agarose (25 ml) | 6His蛋白純化柱料 |
Qiagen 51104 | QIAamp DNA Blood Mini Kit (50) | 血液DNA提取試劑盒 |
Qiagen 51106 | QIAamp DNA Blood Mini Kit (250) | 血液DNA提取試劑盒 |
Qiagen 51183 | QIAamp DNA Blood Midi Kit (20) | 血液DNA提取試劑盒 |
Qiagen 51185 | QIAamp DNA Blood Midi Kit (100) | 血液中提試劑盒 |