人前列環(huán)素(PGI)ELISA 檢測試劑盒,人前列環(huán)素(PGI)ELISA 檢測試劑盒
人前列環(huán)素(PGI)ELISA 檢測試劑盒*試驗(yàn)原理:
ZR試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)(ELISA).已知ZR濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測。先將ZR和生物素標(biāo)記的抗體同時(shí)溫育。洗滌后,加入親和素標(biāo)記過的HRP。再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時(shí)作用。產(chǎn)生顏色。顏色的深淺和樣品中ZR的濃度呈比例關(guān)系。
試劑盒內(nèi)容及其配制
試劑盒成份(2-8℃保存)
96孔配置
48孔配置
配制
96/48人份酶標(biāo)板
1塊板(96T)
半塊板(48T)
即用型
塑料膜板蓋
1塊
半塊
即用型
標(biāo)準(zhǔn)品:40nmol/L
1瓶(0.6ml)
1瓶(0.3ml)
按說明書進(jìn)行稀稀
空白對照
1瓶(1.0ml)
1瓶(0.5ml)
即用型
標(biāo)準(zhǔn)品稀釋緩沖液
1瓶(5ml)
1瓶(2.5ml)
即用型
生物素標(biāo)記的抗ZR抗體
1瓶(6ml)
1瓶(3.0ml)
即用型
親和鏈酶素-HRP
1瓶(10ml)
1瓶(5.0ml)
即用型
洗滌緩沖液
1瓶(20ml)
1瓶(10ml)
按說明書進(jìn)行稀釋
底物A
1瓶(6.0ml)
1瓶(3.0ml)
即用型
底物B
1瓶(6.0ml)
1瓶(3.0ml)
即用型
終止液
1瓶(6.0ml)
1瓶(3.0ml)
即用型
標(biāo)本稀釋液
1瓶(12ml)
1瓶(6.0ml)
即用型
【簡單介紹】
人前列環(huán)素(PGI)ELISA試劑盒"標(biāo)本取樣的基本要求 :
*標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn)。若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融
*不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
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RS11662B 大鼠可溶性白細(xì)胞分化抗原30配體(sCD30L)elisa試劑盒 Rat Soluble Cluster of differentiation 30 ligand,sCD30L Elisa試劑盒
RS11663B 大鼠可溶性白細(xì)胞分化抗原40配體(sCD40L)elisa試劑盒 Rat Soluble Cluster of differentiation 40 ligand,sCD40L Elisa試劑盒
RS11664B 大鼠干細(xì)胞因子受體(SCFR)elisa試劑盒 Rat Stem Cell Factor Receptor,SCFR Elisa試劑盒
RS11665B 大鼠基質(zhì)細(xì)胞衍生因子1β(SDF-1β/CXCL12)elisa試劑盒 Rat Stromal cell derived factor 1β,SDF-1β Elisa試劑盒
RS11666B 大鼠血管生成素受體Tie1(ANG-R-Tie1)elisa試劑盒 Rat angiopoietin receptor Tie1,ANG-R-Tie1 Elisa試劑盒
RS11667B 大鼠血管生成素受體Tie2(ANG-R-Tie2)elisa試劑盒 Rat angiopoietin receptor Tie2,ANG-R-Tie2 Elisa試劑盒
RS11668B 大鼠基質(zhì)金屬蛋白酶抑制因子2(TIMP-2)elisa試劑盒 Rat tissue inhibitors of metalloproteinase 2,TIMP-2 Elisa試劑盒
RS11669B 大鼠基質(zhì)金屬蛋白酶抑制因子3(TIMP-3)elisa試劑盒 Rat tissue inhibitors of metalloproteinase 3,TIMP-3 Elisa試劑盒
RS11670B 大鼠基質(zhì)金屬蛋白酶抑制因子4(TIMP-4)elisa試劑盒 Rat tissue inhibitors of metalloproteinase 4,TIMP-4 Elisa試劑盒
RS11671B 大鼠腫瘤壞死因子可溶性受體Ⅰ(TNFsR-Ⅰ)elisa試劑盒 Rat Tumor necrosis factor soluble receptor Ⅰ,TNFsR-Ⅰ Elisa試劑盒
RS11672B 大鼠腫瘤壞死因子可溶性受體Ⅱ(TNFsR-Ⅱ)elisa試劑盒 Rat Tumor necrosis factor soluble receptor Ⅱ,TNFsR-Ⅱ Elisa試劑盒
RS11673B 大鼠腫瘤壞死因子β(TNF-β)elisa試劑盒 Rat Tumor necrosis factor β,TNF-β Elisa試劑盒
RS11674B 大鼠血小板生成素(TPO)elisa試劑盒 Rat Thrombopoietin,TPO Elisa試劑盒
RS11675B 大鼠腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體1(TRAIL-R1)elisa試劑盒 Rat tumor necrosis factor-related apoptosis-inducing ligand 1,TRAIL R1 Elisa試劑盒
RS11676B 大鼠腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體3(TRAIL-R3)elisa試劑盒 Rat tumor necrosis factor-related apoptosis-inducing ligand 3,TRAIL R3 Elisa試劑盒
RS11677B 大鼠腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體4(TRAIL-R4)elisa試劑盒 Rat tumor necrosis factor-related apoptosis-inducing ligand 4,TRAIL R4 Elisa試劑盒
RS11678B 大鼠可溶性腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體(sTRAIL)elisa試劑盒 Rat soluble tumor necrosis factor-related apoptosis inducing ligand,sTRAIL Elisa試劑盒
RS11679B 大鼠尿激酶型纖溶酶原激活物(uPA)elisa試劑盒 Rat urokinase plasminogen activator,uPA Elisa試劑盒
RS11680B 大鼠尿激酶型纖溶酶原激活物受體(PLAUR/uPAR)elisa試劑盒 Rat Urokinase-type Plasminogen Activator Receptor,PLAUR/uPAR Elisa試劑盒ab12126 Rabbit polyclonal to TROY 50ug 3450
ab12127 Mouse monoclonal [pool] to TTF1 50ul 3450
ab12128 Rabbit polyclonal to USF1 50ul 3450
ab12129 Rabbit polyclonal to Vitamin D Receptor 500ul 3450
ab12131 Rabbit polyclonal to Twilight 100ug 3450
ab12132 Rabbit polyclonal to YY1 100ul 3450
ab12134 Mouse monoclonal [6A920] to IKB alpha 50ug 3450
ab12135 Mouse monoclonal [39A1431] to IKB alpha (phospho S32 + S36) 50ug 3450
ab12136 Rabbit polyclonal to IKB epsilon 50ul 3450
ab12137 Rabbit polyclonal to IKK gamma 500ul 3450
ab12138 Mouse monoclonal [14A231] to IKK alpha 50ug 3450
ab12139 Mouse monoclonal [10AG2] to IKK beta 50ug 3450
ab12140 Mouse monoclonal [10A9B6] to IKK beta 50ug 3450
ab12141 Mouse monoclonal [46B844] to IKK gamma 50ug 3450
ab12142 Mouse monoclonal [72B587] to IKK iota/IKK epsilon 50ug 3450
ab12143 Rabbit polyclonal to IKK iota/IKK epsilon 50ug 3450
ab12145 Rabbit polyclonal to NFkB p65 50ul 3450
ab12146 Rabbit polyclonal to NFkB p65 50ul 3450
ab12147 Rabbit polyclonal to Geminin 50ug 3450
ab12148 Rabbit polyclonal to YB1 100ug 3450
ab12149 Histone H3 peptide 100ug 1725
ab1215 Mouse monoclonal [BB12 + 733] to CD3 + CD8 (Dual color: FITC + PE) 500ul 3450
ab12151 Rabbit polyclonal to STEP / PTPN5 200ul 3450
ab12152 Rabbit polyclonal to HePTP / PTPN7 200ul 3450
ab12153 Rabbit polyclonal to PTPR 200ul 3450
ab12154 Rabbit polyclonal to PTPIA2 / PTPRN 100ug 3450
ab12157 Rabbit polyclonal to Plk1 (phospho T210) 100ug 2826
ab12159 Rabbit polyclonal to Mre11 - ChIP Grade 100ug 3450
ab12161 Rabbit polyclonal to FOXO1A 50ul 3450
ab12162 Rabbit polyclonal to FOXO3A 50ul 3450
ab12163 Rabbit polyclonal to HAT1 50ul 3450
ab12164 Rabbit polyclonal to HAT1 50ul 3450
ab12165 Rabbit polyclonal to SMARCA2 / BRM 100ul 3450
ab12166 Rabbit polyclonal to SMARCA2 / BRM 100ul 3450
ab12167 Rabbit polyclonal to SMARCB1 100ul 3450
ab12168 Mouse monoclonal [HDAC1-21] to HDAC1 100ul 3450
ab12169 Mouse monoclonal [HDAC2-62] to HDAC2 100ul 3450
ab12170 Rabbit polyclonal to HDAC4 50ul 3450
zui重要是安全性
1. 避免直接接觸終止液和底物A、B。一旦接觸到這些液體,請盡快用水沖洗。
2. 實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。
3. 不要用嘴吸取試劑盒里的任何成份。
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