大鼠維生素B12(VB12)ELISA 檢測(cè)試劑盒,大鼠維生素B12(VB12)ELISA 檢測(cè)試劑盒
大鼠維生素B12(VB12)ELISA 檢測(cè)試劑盒*試驗(yàn)原理:
ZR試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)(ELISA).已知ZR濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測(cè)。先將ZR和生物素標(biāo)記的抗體同時(shí)溫育。洗滌后,加入親和素標(biāo)記過的HRP。再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時(shí)作用。產(chǎn)生顏色。顏色的深淺和樣品中ZR的濃度呈比例關(guān)系。
試劑盒內(nèi)容及其配制
試劑盒成份(2-8℃保存)
96孔配置
48孔配置
配制
96/48人份酶標(biāo)板
1塊板(96T)
半塊板(48T)
即用型
塑料膜板蓋
1塊
半塊
即用型
標(biāo)準(zhǔn)品:40nmol/L
1瓶(0.6ml)
1瓶(0.3ml)
按說明書進(jìn)行稀稀
空白對(duì)照
1瓶(1.0ml)
1瓶(0.5ml)
即用型
標(biāo)準(zhǔn)品稀釋緩沖液
1瓶(5ml)
1瓶(2.5ml)
即用型
生物素標(biāo)記的抗ZR抗體
1瓶(6ml)
1瓶(3.0ml)
即用型
親和鏈酶素-HRP
1瓶(10ml)
1瓶(5.0ml)
即用型
洗滌緩沖液
1瓶(20ml)
1瓶(10ml)
按說明書進(jìn)行稀釋
底物A
1瓶(6.0ml)
1瓶(3.0ml)
即用型
底物B
1瓶(6.0ml)
1瓶(3.0ml)
即用型
終止液
1瓶(6.0ml)
1瓶(3.0ml)
即用型
標(biāo)本稀釋液
1瓶(12ml)
1瓶(6.0ml)
即用型
【簡(jiǎn)單介紹】
大鼠維生素B12(VB12)ELISA試劑盒"標(biāo)本取樣的基本要求 :
*標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn)。若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融
*不能檢測(cè)含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
RS13128B 小鼠可溶性白細(xì)胞分化抗原86(B7-2/sCD86)elisa試劑盒 Mouse soluble Cluster of differentiation 86,sCD86 Elisa試劑盒
RS13129B 小鼠低氧誘導(dǎo)因子1α(HIF-1α)elisa試劑盒 Mouse hypoxia-inducible factor 1α,HIF-1α Elisa試劑盒
RS13130B 小鼠胰淀素(Amylin)elisa試劑盒 Mouse Amylin Elisa試劑盒
RS13131B 小鼠白三烯C4(LTC4)elisa試劑盒 Mouse Leukotriene C4,LT-C4 Elisa試劑盒
RS13132B 小鼠細(xì)胞色素C(Cyt-C)elisa試劑盒 Mouse Cytochrome-C,Cyt-C Elisa試劑盒
RS13133B 小鼠血管緊張素Ⅰ(Ang-Ⅰ)elisa試劑盒 Mouse angiotension Ⅰ,Ang-Ⅰ Elisa試劑盒
RS13134B 小鼠黑色素細(xì)胞抗體(MC Ab)elisa試劑盒 Mouse melanocyte antibody,MC Ab Elisa試劑盒
RS13135B 小鼠二肽基肽酶Ⅳ(DPPⅣ)elisa試劑盒 Mouse dipeptidyl peptldase Ⅳ,DPPⅣ Elisa試劑盒
RS13136B 小鼠過敏毒素/補(bǔ)體片斷4a(C4a)elisa試劑盒 Mouse Complement fragment 4a,C4a Elisa試劑盒
RS13137B 小鼠補(bǔ)體片斷5a(C5a)elisa試劑盒 Mouse Complement fragment 5a,C5a Elisa試劑盒
RS13138B 小鼠補(bǔ)體片斷3a(C3a)elisa試劑盒 Mouse Complement fragment 3a,C3a Elisa試劑盒
RS13139B 小鼠卵泡抑素(FS)elisa試劑盒 Mouse Follistatin,FS Elisa試劑盒
RS13140B 小鼠組織蛋白去乙?;福℉D)elisa試劑盒 Mouse Histone Deacetylase,HD Elisa試劑盒
RS13141B 小鼠甲基化酶(Methylase)elisa試劑盒 Mouse Methylase Elisa試劑盒
RS13142B 小鼠抗增殖細(xì)胞核抗原抗體(PCNA)elisa試劑盒 Mouse proliferating cell nuclear antigen antibody,PCNA Elisa試劑盒
RS13143B 小鼠脂蛋白脂酶(LPL)elisa試劑盒 Mouse lipoprotein lipase,LPL Elisa試劑盒
RS13144B 小鼠抗酒石酸酸性磷酸酶5b(TRACP-5b)elisa試劑盒 Mouse tartrate-resistant acid phosphatase 5b,TRACP-5b Elisa試劑盒
RS13145B 小鼠葡萄糖依賴性胰島素釋放多肽(GIP)elisa試劑盒 Mouse Glucose-dependent insulin-releasing polypeptide,GIP Elisa試劑盒
RS13146B 小鼠糖原磷酸化酶同工酶II(GP-II)elisa試劑盒 Mouse Glycogen phosphorylase II,GP-II Elisa試劑盒
RS13147B 小鼠糖原磷酸化酶同工酶MM(GP-MM)elisa試劑盒 Mouse Glycogen phosphorylase MM,GP-MM Elisa試劑盒
RS13148B 小鼠糖原磷酸化酶同工酶BB(GP-BB)elisa試劑盒 Mouse Glycogen phosphorylase BB,GP-BB Elisa試劑盒
RS13149B 小鼠甲狀腺素抗體(TAb)elisa試劑盒 Mouse Thyroxine antibody,TAb Elisa試劑盒
RS13150B 小鼠抗促甲狀腺素受體抗體(TRAb)elisa試劑盒 Mouse thyroid stimulating hormone receptor antidoby,TRAb Elisa試劑盒
RS13151B 小鼠兒茶酚胺(CA)elisa試劑盒 Mouse catecholamine,CA Elisa試劑盒
RS13152B 小鼠心肌營(yíng)養(yǎng)素1(CT-1)elisa試劑盒 Mouse cardiotrophln 1,CT-1 Elisa試劑盒
RS13153B 小鼠抗α-胞襯蛋白抗體IgG/IgA(α-Fodrin IgG/IgA)elisa試劑盒 Mouse anti-alpha-Fodrin IgG/IgA,α-Fodrin IgG/IgA Elisa試劑盒
RS13154B 小鼠脂蛋白α(Lp-α)elisa試劑盒 Mouse lipoprotein α,Lp-α Elisa試劑盒
RS13155B 小鼠凝血因子Ⅷ相關(guān)抗原(FⅧ-Ag)elisa試劑盒 Mouse coagulation factor Ⅷ related antigen,FⅧ-Ag Elisa試劑盒
RS13156B 小鼠凝血因子Ⅸ(FⅨ)elisa試劑盒 Mouse Fcoagulation factor Ⅸ,FⅨ Elisa試劑盒
RS13157B 小鼠凝血因子Ⅹ(FⅩ)elisa試劑盒 Mouse coagulation factor Ⅹ,FⅩ Elisa試劑盒
RS13158B 小鼠血管性血友病因子/瑞斯托霉素輔因子(vWF)elisa試劑盒 Mouse von Willebrand Factor,vWF Elisa試劑盒
RS13159B 小鼠纖溶酶抗纖溶酶復(fù)合物(PAP)elisa試劑盒 Mouse plasmin-antiplasmin complex,PAP Elisa試劑盒
RS13160B 小鼠凝血酶抗凝血酶復(fù)合物(TAT)elisa試劑盒 Mouse thrombin-antithrombin complex,TAT Elisa試劑盒ab27273 Rabbit polyclonal to beta Amyloid, prediluted 7ml 3450
ab27274 Rabbit polyclonal to CD40, prediluted 7ml 3450
ab27276 Rabbit polyclonal to MMP14, prediluted 7ml 3450
ab27278 Rabbit polyclonal to VEGF Receptor 3 100ul 3450
ab2728 Mouse monoclonal [10A12] to Cytohesin 2 100ug 3450
ab2729 Mouse monoclonal [6G11] to Cytohesin 1 + 2 100ug 3450
ab27291 Rabbit polyclonal to Histone H1.5 100ug 3450
ab2730 Mouse monoclonal [AP6] to alpha Adaptin 100ul 3450
ab27303 Rabbit polyclonal to NRG1 type I 100ug 3450
ab2731 Mouse monoclonal [X22] to Clathrin - Membrane Vesicle Marker 100ul 3450
ab27313 Mouse monoclonal [HYB281-05] to Serum Amyloid P 100ug 3450
ab27317 Rabbit polyclonal to XPB 100ug 3450
ab2732 Rabbit polyclonal to mTOR 50ug 3450
ab27325 Mouse monoclonal [23B1] to Glutathione Peroxidase 3 100ul 3450
ab2733 Mouse monoclonal [2G11] to Mannose 6 Phosphate Receptor (Cation independent) - Late Endosome Marker 100ug 3738
ab27338 Mouse monoclonal [B-L2] to CD82 (Phycoerythrin) 100tests 3795
ab2734 Mouse monoclonal [RL1] to Nuclear Pore O-Linked Glycoprotein - Nuclear Pore Marker 100ul 3450
ab2735 Mouse monoclonal [HGAC85] to O-Linked N-Acetylglucosamine 100ug 3450
ab27359 Mouse monoclonal [B-S12] to CD130 (gp130) 100ug 3450
ab2736 Hamster monoclonal [RL31] to Nucleoporin p62 200ul 3450
ab2737 Mouse monoclonal [RL13] to LAP1 200ul 3450
ab2738 Hamster monoclonal [RL29] to LAP2 200ul 3450
ab2739 Mouse monoclonal [RL2] to O-Linked N-Acetylglucosamine 100ul 3450
ab27390 Mouse monoclonal [B-A38] to Syndecan (FITC) 1ml 3450
ab2740 Mouse monoclonal [C7-50] to Hepatitis C Virus Core Antigen 100ug 3450
ab2741 Mouse monoclonal [X36C] to Hepatitis B Virus X antigen 100ug 3450
ab27418 Rabbit monoclonal [629.2A1.53] to TPA Tissue Plasminogen Activator 200ug 3450
ab27419 Rabbit polyclonal to Somatostatin Receptor 1, prediluted 7ml 3450
ab2742 Rat monoclonal [AN1-15] to Androgen Receptor 50ug 3450
ab27422 Rabbit polyclonal to CD105, prediluted 7ml 3450
ab27423 Rabbit polyclonal to uPA Receptor 200ug 3450
ab27424 Rabbit polyclonal to BP1, prediluted 7ml 3450
ab27425 Rabbit polyclonal to BP1 500ul 3450
zui重要是安全性
1. 避免直接接觸終止液和底物A、B。一旦接觸到這些液體,請(qǐng)盡快用水沖洗。
2. 實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。
3. 不要用嘴吸取試劑盒里的任何成份。
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