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相比近年來有長足進(jìn)展的生物樣品純化和晶體X射線衍射技術(shù)，生物大分子晶體的培養(yǎng)依然是一個試驗(yàn)探索的過程，極大程度地依賴于經(jīng)驗(yàn)并帶有較大的盲目性。 蛋白結(jié)晶所需的各種成分，如沉淀劑、鹽、緩沖液體系、有機(jī)溶劑以及各種添加劑，構(gòu)成一個龐大的多維集合。雖然現(xiàn)在市場上有一些商業(yè)化的晶體生長條件篩選盒,但它們只能構(gòu)成一個一個獨(dú)立的小子集, 遠(yuǎn)遠(yuǎn)無法覆蓋非常復(fù)雜、巨大的蛋白結(jié)晶條件集合。
博亞的BioXtal™結(jié)晶條件篩選盒是為蛋白晶體生長條件篩選而特別開發(fā)的一個晶體生長條件組合。 其成分組成是以大量發(fā)表和未發(fā)表的結(jié)晶統(tǒng)計數(shù)據(jù)為基礎(chǔ)發(fā)展而成。它既包含離散矩陣篩選模式也包括系統(tǒng)性篩選模式，既含*效的結(jié)晶條件也含許多其它結(jié)晶條件篩選盒所未有的條件。 擴(kuò)展了篩選范圍從而增大了發(fā)現(xiàn)結(jié)晶條件的幾率。
該試劑盒有96個條件以適合96孔板自動化篩選設(shè)備，pH范圍涵蓋蛋白晶體最易生長范圍4.5-8.5。所使用的化學(xué)試劑來自國標(biāo)*化學(xué)試劑公司（Fluka、Sigma-Aldrich等），并且每種溶液都經(jīng)過0.22μ慮膜過慮滅菌處理。96個條件分別密封在螺旋蓋試管中，每管不少于10毫升，可隨便使用。
在最近進(jìn)行的大量蛋白晶體篩選測試研究中，博亞BioXtal™結(jié)晶條件篩選盒曾結(jié)晶出了超過50%的可結(jié)晶蛋白。被認(rèn)為是高效結(jié)晶條件組合，是一個對現(xiàn)有商業(yè)化的結(jié)晶條件篩選盒的有力補(bǔ)充和發(fā)展。
BioXtalTM結(jié)晶條件篩選試劑盒 | |
1. 0.9M (NH4)2SO4 ,0.1M Na-acetate pH4.5 2. 0.9M (NH4)2SO4 ,0.02M HEPES pH7.5 ,0.7M NaNO3 3. 1.5M (NH4)2SO4 ,0.1M MES pH6.5 4. 1.5M (NH4)2SO4 ,0.1M Bis-tris pH7.0, 0.7%(w/v) Octyl-β-D-glucopyranoside 5. 1.5M (NH4)2SO4 ,0.1M Tris-HCl pH7.5, 0.05M MgCl2 6. 2.0M (NH4)2SO4 ,0.1M Na-citrate pH5.0, 0.5M NaNO3 7. 2.0M (NH4)2SO4 ,0.1M Bis-tris pH5.5 8. 2.0M (NH4)2SO4 ,0.1M Bis-tris pH6.5 9. 2.0M (NH4)2SO4 ,0.02M HEPES pH7.5 10. 2.0M (NH4)2SO4 ,0.1M Tris-HCl pH8.0, 0.02M CaCl2 ,0.5% Octyl-β-D-glucopyranoside 11. 2.0M (NH4)2SO4 ,0.1M Tris-HCl pH8.5 12. 2.0M (NH4)2SO4 13. 2.88M (NH4)2SO4 ,0.1M Na-citrate pH5.0 14. 2.88M (NH4)2SO4 ,0.02M HEPES pH7.5 15. 15.0%(v/v) Jeffamine M600 ,0.1M HEPES pH7.0 16. 30.0%(w/v) Jeffamine ED-2001 ,0.1M HEPES pH7.0 17. 1.8M Li2SO4 ,0.05M Na Cacodylate pH6.0, 0.01M MgSO4 18. 2.0M Li2SO4 ,0.05M MES pH5.6 ,0.01M MgCl2 19. 15.0%(w/v) PEG 2000 MME ,0.1M Tris-HCl pH7.5 , 0.2M KCl 20. 20.0%(w/v) PEG 2000 MME ,0.1M Tris-HCl pH8.5, 0.2M trimethylamine n-oxide 21. 28.0%(w/v) PEG 2000 MME ,0.1M Bis-tris pH6.5 22. 30.0%(w/v) PEG 2000 MME , 0.05M Na Cacodylate pH6.0 ,0.05M MgCl2 23. 30.0%(w/v) PEG 2000 MME ,0.15M KBr 24. 20.0%(w/v) PEG 5000 MME ,0.1M Bis-tris pH6.5 25. 20.0%(w/v) PEG 5000 MME ,0.1M HEPES pH7.0, 0.5M NaNO3 26. 25.0%(w/v) PEG 5000 MME ,0.1M Tris-HCl pH7.5 27. 25.0%(v/v) PEG 550 MME ,0.1M Na-citrate pH5.0 28. 25.0%(v/v) PEG 550 MME ,0.1M Tris-HCl pH8.5 29. 30.0%(v/v) PEG 550 MME ,0.1M Bis-tris pH7.0 , 0.05M MgCl2 30. 10.0%(v/v) MPD ,0.1M Na-citrate pH5.0 31. 10.0%(v/v) MPD ,0.1M Tris-HCl pH7.5 0.1M CaCl2 32. 20.0%(v/v) MPD ,0.05M Na Cacodylate pH6.0 33. 25.0%(v/v) MPD ,0.1M Na-citrate pH5.0 34. 25.0%(v/v) MPD ,0.1M MES pH6.5 ,0.75M NaNO3 35. 25.0%(v/v) MPD ,0.02M HEPES pH7.5 ,0.05M MgCl2 36. 30.0%(v/v) MPD ,0.1M Na-acetate pH4.5 37. 30.0%(v/v) MPD ,0.1M MES 6.5 ,0.02M CaCl2 38. 30.0%(v/v) MPD ,0.1M Tris-HCl pH8.5 ,0.02M MgCl2 39. 40.0%(v/v) MPD ,0.01M CaCl2 40. 10.0%(v/v) MPD ,0.1M Tris-HCl pH7.5 ,0.01M MgCl2 , 0.05M NH4-acetate 41. 1.2M NaCl ,0.1M MES pH6.5 42. 1.2M NaCl ,0.6M NaNO3 , 0.25%(w/v) Octyl-β-D-glucopyranoside 43. 1.5M NaCl ,0.1M Na-acetate pH4.5 44. 1.5M NaCl ,0.1M Bis-tris pH7.0 45. 1.5M NaCl ,0.1M Tris-HCl pH8.5 ,0.01M CaCl2 , 0.4%(w/v) Octyl-β-D-glucopyranoside 46. 1.5M NaCl ,0.1M Tris-HCl pH8.5 ,0.6M NaNO3 47. 1.5M NaCl 0.1M NH4-acetate , 0.4% Octyl-β-D-glucopyranoside 48. 2.0M NaCl ,0.02M HEPES 7.5 49. 2.0M NaCl ,0.3M NaNO3 50. 2.5M NaCl ,0.1M Na-citrate pH5.0 , 0.9M NaNO3 51. 2.5M NaCl ,0.1M Bis-tris pH6.5 ,0.3M NaNO3 52. 2.5M NaCl ,0.1M Bis-tris pH7.0 , 0.1M NH4-acetate |
53. 2.5M NaCl ,0.02M HEPES pH7.5 ,0.05M MgCl2 54. 2.5M NaCl ,0.02M HEPES pH7.5 55. 2.9M NaCl ,0.1M Tris-HCl pH8.5, 0.4%(w/v) Octyl-β-D-glucopyranoside 56. 3.0M NaCl ,0.1M Na-citrate pH5.0 ,0.5M NaNO3 57. 3.1M NaCl ,0.1M Bis-tris pH7.0 58. 3.5M NaCl ,0.1M Bis-tris pH5.5 59. 3.5M NaCl ,0.1M Tris-HCl pH7.5 ,0.05M MgCl2 60. 30.0%(w/v) PEG 1000 ,0.1M Tris-HCl pH7.5 , 5.0%(v/v) Glycerol 61. 17.0%(w/v) PEG 10000 ,0.1M Bis-tris pH5.5 , 0.1M NH4-acetate 62. 15.0%(w/v) PEG 3350 ,0.1M MES pH6.5 ,0.85M NaNO3 63. 17.0%(w/v) PEG 3350 ,0.1M Tris-HCl pH8.5 64. 20.0%(w/v) PEG 3350 ,0.02M HEPES pH7.5 , 0.05M MgCl2 65. 20.0%(w/v) PEG 3350 ,0.2M Na-formate 66. 21.0%(w/v) PEG 3350 ,0.02M HEPES pH7.5, 0.7% Octyl-β-D-glucopyranoside 67. 22.0%(w/v) PEG 3350 ,0.1M MES pH6.5 ,0.08M CaCl2 , 0.7% Octyl-β-D-glucopyranoside 68. 24.0%(w/v) PEG 3350 ,0.01M CaCl2 69. 25.0%(w/v) PEG 3350 ,0.1M Na-citrate pH5.0 70. 25.0%(w/v) PEG 3350 ,0.1M Bis-tris pH5.5 , 0.2M Li2SO4 71. 25.0%(w/v) PEG 3350 ,0.1M Bis-tris pH5.5, 0.2M NaCl 72. 25.0%(w/v) PEG 3350 ,0.1M Bis-tris pH5.5 73. 25.0%(w/v) PEG 3350 ,0.1M Bis-tris pH5.5, 0.2M NH4-acetate 74. 25.0%(w/v) PEG 3350 ,0.1M Bis-tris pH5.5 , 0.2M MgCl2 75. 25.0%(w/v) PEG 3350 ,0.1M Bis-tris pH6.5 , 0.2M MgCl2 76. 25.0%(w/v) PEG 3350 ,0.1M Bis-tris pH6.5 , 0.2M NH4-acetate 77. 25.0%(w/v) PEG 3350 ,0.1M HEPES pH7.5 , 0.2M MgCl2 78. 25.0%(w/v) PEG 3350 ,0.1M HEPES pH7.5 ,0.2M NaCl 79. 25.0%(w/v) PEG 3350 ,0.1M HEPES pH7.5 80. 25.0%(w/v) PEG 3350 ,0.1M HEPES pH7.5 , 0.2M NH4-acetate 81. 25.0%(w/v) PEG 3350 ,0.1M HEPES pH7.5, 0.2M (NH4)2SO4 82. 25.0%(w/v) PEG 3350 ,0.1M Tris-HCl pH8.5 , 0.2M NaCl 83. 25.0%(w/v) PEG 3350 ,0.1M Tris-HCl pH8.5 84. 25.0%(w/v) PEG 3350 ,0.1M Tris-HCl pH8.5 , 0.2M NH4-acetate 85. 25.0%(w/v) PEG 3350 ,0.1M Tris-HCl pH8.5 , 0.2M MgCl2 86. 28.0%(w/v) PEG 3350 ,0.1M Bis-tris pH 7.0 , 0.02M CaCl2 87. 30.0%(w/v) PEG 3350 ,0.1M Na-citrate pH5.0 88. 30.0%(w/v) PEG 3350 ,0.4M NaNO3 89. 40.0%(w/v) PEG 3350 ,0.1M Na-citrate pH5.0 90. 40.0%(w/v) PEG 3350 ,0.1M Tris-HCl pH7.5 , 0.4M NaNO3 91. 30.0%(v/v) PEG 400 ,0.05M Na Cacodylate pH6.0 92. 30.0%(v/v) PEG 400 ,0.1M HEPES pH7.5 ,0.2M MgCl2 93. 35.0%(v/v) PEG 400 ,0.1M Bis-tris pH6.5 , 0.1M NH4-acetate 94. 25.0%(w/v) PEG 4000 ,0.1M Na-acetate pH4.6 , 0.2M (NH4)2SO4 95. 30.0%(v/v) pentaerythritol ethoxylate (15/4 EO/OH) , 0.05M Bis-tris pH6.5 ,0.05M (NH4)2SO4 96. 35.0%(v/v) pentaerythritol propoxylate (5/4 PO/OH) , 0.05M HEPES pH7.5 ,0.2M KCl |
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