說明書下載SLLU.rar分類名稱 : 高效藥物篩選 產(chǎn)品介紹 : 測定方法 快速(2分鐘內(nèi))生物發(fā)光法測定熒光素酶報(bào)告基因在細(xì)胞中的表達(dá)。 Description The SuperLightTM Luciferase Reporter Gene Assay is based on the quantitation of luciferase expression in mammalian, yeast or E. coil cells, using luciferin and ATP as substrates. The reaction results in light production which can be conveniently measured on a luminometer Reaction scheme:This bioluminescent reporter gene assay is extremely sensitive and is especially suitable for quantifying luciferase expression in recombinant cells. This ultra-sensitive, homogeneous cell-based assay only requires adding a single reagent to the cells and measuring the light intensity after a short incubation step (2 minutes). Assays can be performed in tubes, cuvettes or multi-well plates. All kit components are compatible with culture media and with all liquid handling systems. With an extended luminescence emission kinetics (half-life 40 min), the SuperLightTM luciferase assays are especially suitable for high-throughput screening in 96-well, 384-well and 1536-well plates. In addition, the reagent provided in the kits has been formulated for maximum sensitivity, reproducibility and long shelf-life. Applications for this kiinclude gene t regulation studies and high-throughput screening of gene modulators. Key features High sensitivity and wide detection range: detection of as little of 2 fg luciferase and as few as 4 cells. Plus, the emitted light is linear over seven orders of magnitude.Compatible with routine laboratory and HTS formats: assays can be performed in tubes or microplates, on LJL Analyst, Berthold Luminometer, Top-Count, MicroBeta counters, chemiluminescent image plate readers (CLIPR/LeadSeeker). Assay reagents compatible with all liquid handling systems.Fast and convenient: homogeneous “mix-and-measure” assay allows detection of luciferase levels within 10 minutes. The optimally combined reagent system allows a single addition step, and simultaneous cell lysis and detection.Robust and amenable to HTS: Z’ factors of 0.6 to 0.8 are observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems. Applications Gene Regulation: gene expression level, characterization of promoter activity, modulation of gene expression by receptors, transcription factors and small molecules.Drug Discovery: high-throughput screen for gene modulators. Storage conditions. Store the Reagent in the provided amber tube at -20°C and the Assay Buffer at 2-8°C. Shelf life: 12 month.
其他 : 編號:SLLU-048,SLLU-200 測試:48,200T |
北京鴻躍創(chuàng)新科技有限公司是一家經(jīng)營科研產(chǎn)品代理、銷售生物試劑、生物耗材、生物儀器等于一身的生物技術(shù)企業(yè),其產(chǎn)品覆蓋了生命領(lǐng)域從分子生物學(xué)研究、蛋白表達(dá)、細(xì)胞培養(yǎng)到層析純化,從抗原抗體、成套試劑盒到生物培養(yǎng)及其生物活性研究等相關(guān)試劑幾近所有過程.
在生物公司林立的今天,鴻躍公司始終堅(jiān)持以“*,信譽(yù)*”的服務(wù)宗旨.憑借其對市場的充分了解,對產(chǎn)品深入細(xì)致的分析,以其雄厚的資本,快速的流通方式,隨時(shí)為客戶提供*、技術(shù)含量高、操作方便快捷的實(shí)驗(yàn)急需產(chǎn)品.
鴻躍公司在為廣大科學(xué)工作者們提供產(chǎn)品的同時(shí)也注重公司員工自身的素質(zhì)培養(yǎng).不但要求員工有扎實(shí)的專業(yè)基礎(chǔ),還要有較高的文化素質(zhì)和道德修養(yǎng).強(qiáng)調(diào)員工與客戶建立良好的合作關(guān)系,公司內(nèi)部的團(tuán)隊(duì)合作精神.公司規(guī)模不求較大,但求Z佳,價(jià)格不求Z低,但求合理.
總之,鴻躍公司將以“更加執(zhí)著地追求客戶的滿意度”為宗旨,全心全意地為客戶做好每一件事.同是,對一直以來關(guān)心鴻躍公司的朋友們深表感謝!讓我們與生命科學(xué)攜手共步美好的明天!
經(jīng)營范圍
現(xiàn)貨提供兩千多種包括Sigma、Aldrich、Fluka、 Amresco、Merck、Roche、Serva、Promega、Pharmacia、 Cambrex(BioWhittaker)、Gibco、HyClone、BMA、Qbiogene、Oxid、BioLabs、Acros、Difco、等國外*公司的產(chǎn)品,并承擔(dān)以上公司專項(xiàng)訂貨業(yè)務(wù)。
二.分子生物學(xué)成套試劑盒
現(xiàn)貨提供美國Omega、Qbiogene(Bio101)、Promega核酸分離和純化試劑盒,自產(chǎn)試劑盒。期貨提供德國Qiagen、MoBiTec、美國Ambion試劑盒。
三.免疫學(xué)試劑及檢測試劑盒
*現(xiàn)貨提供三千多種Sigma、R&D、Zymail、UsBio、Santa Curz、Maixin-Bio進(jìn)口分裝抗體及免疫組化配套試劑。Biosoure、R&D 、Diaclone等公司進(jìn)口分裝Elisa Kit、Eli-spot Kit。Biotium分子探針。各種國產(chǎn)及進(jìn)口分裝二抗,國產(chǎn)檢測試劑盒及細(xì)胞因子等。
期貨提供產(chǎn)品包括Santa Cruz、Calbiochem等公司的大包裝抗體和 Maixin-Bio、Affiniti、Neomarkers、Genx BioSciense、Zyman、UsBio等公司的抗體;KPL、Santa Cruz等公司的二抗;Maixin-Bio等公司的免疫組化配套試劑;Diaclone、 IBL、 MBL、Endogen、DSL、Biosource等公司的Elisa Kit;Diaclone公司的Eli-spot Kit;Biotium、MoiecularProbe公司探針;Peprotech,、R&D、Sigma、Calbiochem等公司的細(xì)胞因子。
四.細(xì)胞生物學(xué)產(chǎn)品
現(xiàn)貨部分提供美國Gibco、HyClone、Oxoid公司的培養(yǎng)基、血清、等培養(yǎng)試劑。并承擔(dān)以上公司專項(xiàng)訂貨業(yè)務(wù)。
五.試驗(yàn)耗材
現(xiàn)貨提供德國Sartorius,英國Whatman等過濾及超濾產(chǎn)品?,F(xiàn)貨提供美國Corning Costar、B.D、日本IWAKI、比利時(shí)Orange、丹麥NUCK、德國Greiner等細(xì)胞培養(yǎng)耗材?,F(xiàn)貨提供美國Axygen、Qsp、德國Brand、SCHOTT、芬蘭Biohit、美國Fisher等常規(guī)耗材?,F(xiàn)貨提供各種國產(chǎn)耗材。
六.實(shí)驗(yàn)儀器及設(shè)備
現(xiàn)貨提供進(jìn)口原裝芬蘭百得(Biohit),雷勃,大龍移液產(chǎn)品;德國Brand、Eppendorf;法國Gilson;日本Nichiryo等國移液設(shè)備。提供各種進(jìn)口及國產(chǎn)實(shí)驗(yàn)儀器及設(shè)備。
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產(chǎn)品名稱參考價(jià)