齊一生物黑色素細胞刺激素(MSH)ELISA檢測試劑盒齊一生物科技有限公司新品*,齊一生物科技有限公司長期經(jīng)營大鼠ELISA試劑盒,人elisa試劑盒,小鼠elisa試劑盒等種屬試劑盒,歡迎您訪問我們的,www.qiyibio。。com全國:歡迎選購!如有什么問題請給我們或者留言,告訴我您的需要,并留下您寶貴的意見和建議
齊一生物黑色素細胞刺激素(MSH)ELISA檢測試劑盒類別:酶聯(lián)免疫分析檢測試劑盒
規(guī)格:96T/48T
包裝:盒
檢測原理:采用雙抗夾心法
保質(zhì)期:六個月
產(chǎn)品保存條件:2-8°C
應(yīng)用范圍:僅供科研研究實驗使用
樣品形式:血清/血漿/組織勻漿/細胞培養(yǎng)上清//其它生物液體(具體情況請咨詢)
標本的采集及保存:
1.血清:室溫血液自然凝固10-20分鐘后,離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細收集上清。保存 過程 中 如有沉淀形成,應(yīng)再次離心。
2.血漿:應(yīng)根據(jù)標本的要求選擇EDTA、檸檬酸鈉或肝素作為抗凝劑,混合10-20分鐘后,離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細收集上清。保存過程中如有沉淀形成,應(yīng)再次離心。
3.尿液:用無菌管收集。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細收集上清。保存過程中如有沉淀形成,應(yīng)再次離心。胸腹水、腦脊液參照此實行。
4.細胞培養(yǎng)上清:檢測分泌性的成份時,用無菌管收集。離心20分鐘左右(2000-3000轉(zhuǎn)/ 分)。仔細收集上清。 檢測細胞內(nèi)的成份時,用PBS(PH7.2-7.4)稀釋細胞懸液,細胞濃度達到100萬/ml左右。通過反復凍融,以 使細胞破壞并 放出細胞內(nèi)成份。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細收集上清。保存過程中如有沉淀形成,應(yīng)再次離心。
5.組織標本:切割標本后,稱取重量。加入一定量的PBS,PH7.4。用液氮迅速冷凍保存?zhèn)溆?。標本融化后仍然保?-8℃的溫度。加入一定量的PBS(PH7.4),用手工或勻漿器將標本勻漿充分。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細收集上清。分裝后一份待檢測,其余冷凍備用。
標本要求:
1.標本采集后盡早進行提取,提取按相關(guān)文獻進行,提取后應(yīng)盡快進行實驗。若不能馬上進行試驗,可將標本放于-20℃保存,但應(yīng)避免反復凍融
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PA516291 GFAP 500 ul
PA516301 Anti-CD31 500 ul
PA516305 Anti-HER-2 500 ul
PA516310 GRP94/HSP90B1 500 ul
PA516318 Anti-COX-1 500 ul
PA516322 Anti-Topoisomerase II alpha 500 ul
PA516327 HSP90/Heat Shock Protein 90 beta 500 ul
PA516332 Anti-Caspase 3 500 ul
PA516335 Anti-Caspase 3 500 ul
PA516341 HSP90/Heat Shock Protein 90 alpha 500 ul
PA516355 Anti-Caspase 9 500 ul
PA516358 Anti-Caspase 9 500 ul
PA516363 Anti-Androgen Receptor 500 ul
PA516368 Anti-Hepatitis B Virus Core A ntigen 500 ul
PA516376 Anti-Herpes Simplex Virus Typ e 2 500 ul
PA516380 Anti-TAU 500 ul
PA516388 Anti-Human IgA (alpha-Heavy C hain) 500 ul
PA516396 Anti-Human IgE (epsilon-Heavy Chain) 500 ul
PA516400 Anti-Human IgD (delta-Heavy C hain) 500 ul
PA516409 Anti-Gastrin 500 ul
PA516413 MBP 500 ul
PA516417 Anti-Synaptophysin 500 ul
PA516423 Anti-Factor XIII A 500 ul
PA516429 beta Catenin 500 ul
PA516435 Anti-Progesterone Receptor 500 ul
PA516440 Anti-Estrogen Receptor 500 ul
PA516446 Anti-Ki-67 500 ul
PA516452 Anti-PARP (Poly ADP-Ribose Po lymerase) 500 ul
PA516458 Anti-CD117 500 ul
PA516464 Anti-Calcitonin 500 ul
PA516470 Anti-Bak 500 ul
PA516476 Anti-Estrogen Receptor 500 ul
PA516481 Anti-Cadherin Pan 500 ul
PA516487 Anti-Flk-1 500 ul
PA516493 Anti-Flt-1 500 ul
PA516498 Anti-MMP-1 (Collagenase-1) 500 ul
PA516504 Anti-MMP-2 (72kDa Collagenase IV) 500 ul
PA516509 Anti-MMP-9 (92kDa Collagenase IV) 500 ul
PA516514 Anti-MMP-14 500 ul
PA516519 Anti-Cyclin A 500 ul
PA516524 Anti-iNOS 500 ul
PA516529 Anti-CXCR4 500 ul
PA516534 Anti-Survivin 500 ul
PA516539 Anti-p57Kip2 500 ul
PA516545 Anti-NFkappaB/p65 500 ul
PA516551 Anti-TNF-alpha converting enz yme 500 ul
PA516556 Anti-MEK-1 500 ul
PA516561 Anti-PARP (Poly ADP-Ribose Po lymerase) 500 ul
PA516566 Anti-MMP-13 (Collagenase-3) 500 ul
PA516571 Anti-PDGFR alpha 500 ul
PA516576 Anti-PDGFR beta 500 ul
PA516581 Anti-Gamma-Glutamyl cysteine Synthetase/Glutamate-cysteine 500 ul
PA516586 Anti-S100 A4 500 ul
PA516590 Anti-S100 A6 500 ul
PA516598 Pierce Rabbit anti-Human IgM Secondary Antibody, FITC 2 ml
PA516599 Anti-Fibrinogen 2 ml
PA516600 Anti-Albumin 2 ml
PA516601 Anti-C1q Complement 2 ml
PA516602 Anti-C4 Complement 2 ml
PA516603 Anti-Kappa Light Chain 2 ml
PA516604 Anti-Lambda 2 ml
PA516607 Anti-Cyclin D1/Bcl-1 500 ul
PA516616 Anti-Amphiregulin 500 ul
PA516621 Anti-Amphiregulin 500 ul
PA516626 Anti-Heregulin 500 ul
PA516629 Anti-Heregulin 500 ul
PA516634 Anti-von Willebrand Factor 500 ul
PA516638 Anti-Toxoplasma Gondii 500 ul
PA516642 Anti-HER-4 500 ul
PA516647 Anti-Kappa Light Chain 500 ul
PA516651 Anti-Lambda Light Chain 500 ul
PA516655 Anti-CD3epsilon 500 ul
PA516658 Anti-Alpha Fetoprotein 500 ul
PA516661 Anti-Alpha-1-Antitrypsin 500 ul
PA516665 Anti-CD66e 500 ul
PA516668 Anti-Lysozyme 500 ul
PA516672 Anti-Myeloperoxidase 500 ul
PA516676 Anti-FAK-Focal Adhesion Kinase 500 ul
PA516681 Anti-Calretinin 500 ul
PA516685 Anti-Chromogranin A 500 ul
PA516693 Anti-CD8 500 ul
PA516697 Anti-Actin Smooth Muscle 500 ul
PA516701 Anti-CD20 500 ul
PA516705 Anti-Desmin 500 ul
PA516709 Anti-Progesterone Receptor 500 ul
PA516713 Anti-S100 500 ul
PA516717 Anti-p27Kip1 500 ul
PA516722 Anti-Cytokeratin 14 500 ul
PA516726 Anti-Cytokeratin 19 500 ul
PA516730 Anti-Amyloid Precursor Protei n (APP) 500 ul