雌二醇檢測(cè)試劑盒(血清)齊一生物長(zhǎng)期經(jīng)營ELISA試劑盒(免費(fèi)代測(cè))及抗體,分子學(xué)生物試劑盒,金標(biāo)法檢測(cè)試劑盒,比色法檢測(cè)試劑盒,細(xì)胞菌株,培養(yǎng)基,細(xì)胞因子,生化試劑,耗材等生物試劑產(chǎn)品.誠信經(jīng)營,價(jià)格實(shí)惠,服務(wù)周到,質(zhì)量保障!提供各種實(shí)驗(yàn)代測(cè)服務(wù).歡迎新老客戶!齊一生物科技(上海)有限公司,專業(yè)生產(chǎn)研發(fā)生化科研試劑全國:
雌二醇檢測(cè)試劑盒(血清)有效期:6個(gè)月
預(yù)期應(yīng)用:ELISA法定量測(cè)定血清、血漿、細(xì)胞培養(yǎng)上清或其它相關(guān)生物液體中的含量.
說明
1.試劑盒保存:-20℃(較長(zhǎng)時(shí)間不用時(shí));2-8℃(頻繁使用時(shí)).
2.濃洗滌液低溫保存會(huì)有鹽析出,稀釋時(shí)可在水浴中加溫助溶.
3.中、英文說明書可能會(huì)有不*之處,請(qǐng)以英文說明書為準(zhǔn).
4.剛開啟的酶聯(lián)板孔中可能會(huì)含有少許水樣物質(zhì),此為正常現(xiàn)象,不會(huì)對(duì)實(shí)驗(yàn)結(jié)果造成任何影響.
實(shí)驗(yàn)原理
用純化的抗體包被微孔板,制成固相載體,往包被抗該指標(biāo)抗體的微孔中依次加入標(biāo)本或標(biāo)準(zhǔn)品、生物素化的抗該指標(biāo)抗體、HRP標(biāo)記的親和素,經(jīng)過*洗滌后用底物TMB顯色.TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成zui終的黃色.顏色的深淺和樣品中的該指標(biāo)呈正相關(guān).用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度(OD值),計(jì)算樣品濃度.
試劑盒組成及試劑配制
1. 酶聯(lián)板(Assay plate ):一塊(96孔).
2. 標(biāo)準(zhǔn)品(Standard):2瓶(凍干品).
3. 樣品稀釋液(Sample Diluent):1×20ml/瓶.
4. 生物素標(biāo)記抗體稀釋液(Biotin-antibody Diluent):1×10ml/瓶.
5. 辣根過氧化物酶標(biāo)記親和素稀釋液 (HRP-avidin Diluent):1×10ml/瓶.
6. 生物素標(biāo)記抗體(Biotin-antibody):1×120μl/瓶(1:100)
7. 辣根過氧化物酶標(biāo)記親和素(HRP-avidin):1×120μl/瓶(1:100)
8. 底物溶液(TMB Substrate):1×10ml/瓶.
9. 濃洗滌液(Wash Buffer):1×20ml/瓶,使用時(shí)每瓶用蒸餾水稀釋25倍.
10. 終止液(Stop Solution):1×10ml/瓶(2N H2SO4).
HU MIP-1 ALP NHP APC MAB 50TST 11A3 551533
HU CMRF-56 PURE MAB 0.1MG CMRF-56 551534
PIG CD45RC PURE MAB 0.1MG 3A56 551536
MS CD4 IMAG DM 10ML GK1.5 551539
PIG GMA DTA T LYM PURE MAB 0.1MG MAC320 551543
HU CD209 APC MAB 100TST DCN46 551545
MS H2-M3 PURE MAB 0.1MG 130 551769
RAT MAST CELLS PURE MAB 0.1MG AR32AA4 551770
MS DO-11.10 PURE MAB 0.25MG KJ1-26 551771
MS DO-11.10 PE MAB 0.1MG KJ1-26 551772
HU CD196 (CCR6) NHP PE MAB 50TST 11A9 551773
HU IL-4 NHP PE MAB 50TST 8D4-8 551774
HU CD66C FITC MAB 100TST B6.2/CD66 551775
MS DNDRTC CELLS PURE MAB 0.5MG 33D1 551776
RBT CD25 PURE MAB 0.1MG KEI-ALP 1 551779
HU IFN-ALP NALE MAB 0.5MG 7N4-1 551795
HU FETAL HB PURE MAB 0.1MG 2D12 551796
RAT IGG2A KPA ITCL NHP PE 50TST R35-95 551799
RAT GMA DTA TCR PE MAB 0.1MG V65 551802
RAT CD42D FITC MAB 0.1MG RPM.4 551805
RAT CD9 PURE MAB 0.1MG RPM.7 551808
HU TH1-TH2 CBA KIT II 551809
HU TH1-TH2 CBA STD 0.2ML LYO 551810
HU INFLAMMATORY CK CBA KIT 551811
POLY ADP-RIBOSE PAB W/CONT 0.1ML LP96-10 551813
DSRED PURE MAB W/CONT 50UG E64-1077 551814
HU SYK PURE MAB W/CONT 50UG 4D10 551816
HU SYK PURE MAB W/CONT 150UG 4D10 551817
HU IKBA PHOSPHO-SPEC PURE 50UG 39A1413 551818
HU IKBA PURE MAB W/CONT 50UG 6A920 551819
HU IFN-GMA BD ELISPOT SET 10PLT 551849
HU CD126 PE MAB 100TST M5 551850
MS CD184 PURE MAB 0.1MG 2B11/CXCR4 551852
MS CD124 PURE MAB 0.1MG MIL4R-M1 551853
MS PRE-BCR BIOTIN MAB 0.1MG SL156 551863
MS CD179B LAM 5 BIOTIN MAB 0.1MG LM34 551865
MS IFN-GMA OPTEIA SET 20TST 551866
HU IFN-GMA ELISPOT PAIR 551873
MS TNF ELISPOT PAIR 551875
MS IL-2 ELISPOT PAIR 551876
MS IL-4 ELISPOT PAIR 551878
MS IL-5 ELISPOT PAIR 551880
MS IFN-GMA ELISPOT PAIR 551881
HU TNF ELISPOT PAIR 551882
HU IL-10 ELISPOT PAIR 551883
HU IL-2 ELISPOT PAIR 551884
HU IL-4 ELISPOT PAIR 551885
HU IL-5 ELISPOT PAIR 551886
HU CD180 PURE MAB 0.1MG G28-8 551890
MS CD279 PURE MAB 0.1MG J43 551891
Clamp Bracket, Y-arm mounting, Cxx 9019567 qiagen
Pin, lid hinge, CAS 9019549 qiagen
Plate Grippers RD, RCS 9019522 qiagen
miScript II RT Kit (50) 218161 qiagen
miScript SYBR Green PCR Kit (1000) 218075 qiagen
Hinge, front rear hinge plates, RG3 9019430 qiagen
Solenoid, lid lock, RG3 9019398 qiagen
Fan, 120mm, rear panel, RG3 9019395 qiagen
Board, main, standard, RG3 9019383 qiagen
Wiring, lower heater, RG6 9019366 qiagen
Solenoid, lid lock assembly, RG6 9019365 qiagen
Sensor, heater fan speed, RG6 9019349 qiagen
AllStars Neg. siRNA AF 488 (20 nmol) 1027292 qiagen
Switch - ON/OFF, PyroQ24 9019240 qiagen
Manual WinRufas 2.5 (CD) 9234698 qiagen
QuantiFast SYBR Green PCR Kit (4000) 204057 qiagen
Switch, micro, GSC 9020475 qiagen
Bush, insulation front, GSC 9020474 qiagen
Mirror, 10 x 10 x 2 mm, GS 9020472 qiagen
System Liquid Bottle (Rotor) 9234469 qiagen
Coupling, CPC, inline, female, 6mm 9234461 qiagen
Boundary, SIS, left 9234434 qiagen
Pump, dispenser, BR8000 9234385 qiagen
AllStars Neg. siRNA AF 647 (5 nmol) 1027287 qiagen
Gasket, cooling flap, RG6 9019816 qiagen
Service kit, 1 channel pipettor, CAS1200 9019815 qiagen
Upgrade Package 1, RCS 9019803 qiagen
Maintenance kit, annual, BR9600 (D) 9013436 qiagen
Plate holder for rotor and bucket 9238810 qiagen
PCB, lge breakout, 12 core to rev 3, Cx2 9019624 qiagen
Cover, Pipettor, 1 ch. (white), CAS 9019610 qiagen
Bracket, X-axis opto, Cxx 9019561 qiagen
Power entry port, Cxx 9019539 qiagen
BLOCK LOADING 80 WELL COM ASSY 9019523 qiagen
RISER VACUUM 20MM 9019507 qiagen
FastLane Cell SYBR Green Kit (200) 216213 qiagen
Rotor-Gene SYBR Green PCR Kit (2000) 204076 qiagen
Board, distribution incl. PTCs, RG3 9019405 qiagen
LED, green (530), RG3 9019388 qiagen
Filter, fuse, switch mains power, RGx 9019359 qiagen
Sensor, optics source home, RG6 9019344 qiagen
LED, IR, RG6 9019342 qiagen
Mikrowin Kinetic Software 9234701 qiagen
Cover, front, GSC 9020470 qiagen
Fuse T6,3A 9234663 qiagen
AllStars Neg. siRNA AF 488 (5 nmol) 1027284 qiagen
miScript Negative Control Inhititor(20) 1027272 qiagen
Belt, tooth, handler z, BR8000 9233431 qiagen
Penicillin-Streptomycin,Liquid 100ml
20ml
100ml
20*100ml
Antibiotic Mixture 100ml
Antibiotic-Antimycotic (100X) 20ml
100ml
20*100ml
Pen-Stre-Glu (100X) 100ml
Gentamicin Reagent Solution(10mg/ml),Liquid 10ml
10*10ml
ZEOCIN 1g
Geneticin 1g
5g
25g
產(chǎn)品名稱 規(guī)格
DPBS(1X) 100ml
500ml
10x500ml
1000ml
6x1000ml
DPBS(1X) 500ml
10x500ml
1000ml
6x1000ml
DPBS(1X) 500ml
1000ml
DPBS(10X) 500ml
DPBS(10X) 500ml
10x500ml
HBSS(1X) 500ml
10x500ml
1000ml
6x1000ml
HBSS(1X) 100ml
500ml
10x500ml
HBSS(1X) 500ml
10x500ml
1000ml
6x1000ml
HBSS(1X) 500ml
HBSS(10X) 500ml
HBSS(10X) 500ml