α乳清蛋白(α-La)檢測(cè)試劑盒齊一生物科技(上海)有限公司專業(yè)經(jīng)營(yíng)ELISA試劑盒種類齊全,到貨快、確保每一個(gè)ELISA試劑盒質(zhì)量。凡是購(gòu)買我公司ELISA試劑盒免費(fèi)代測(cè)、提供技術(shù)服務(wù)、5個(gè)工作日出實(shí)驗(yàn)結(jié)果。歡迎新老客戶前來(lái): 到貨快捷安全:專業(yè)的物流配送,專人全程跟蹤訂單,確保貨物安全、準(zhǔn)確、及時(shí)地送達(dá)地點(diǎn)
α乳清蛋白(α-La)檢測(cè)試劑盒應(yīng)用范圍:僅供科研研究實(shí)驗(yàn)使用
樣品形式:血清/血漿/組織勻漿/細(xì)胞培養(yǎng)上清//其它生物液體(具體情況請(qǐng)咨詢)
標(biāo)本的采集及保存:
1.血清:室溫血液自然凝固10-20分鐘后,離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。保存 過(guò)程 中 如有沉淀形成,應(yīng)再次離心。
2.血漿:應(yīng)根據(jù)標(biāo)本的要求選擇EDTA、檸檬酸鈉或肝素作為抗凝劑,混合10-20分鐘后,離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。保存過(guò)程中如有沉淀形成,應(yīng)再次離心。
3.尿液:用無(wú)菌管收集。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。保存過(guò)程中如有沉淀形成,應(yīng)再次離心。胸腹水、腦脊液參照此實(shí)行。
4.細(xì)胞培養(yǎng)上清:檢測(cè)分泌性的成份時(shí),用無(wú)菌管收集。離心20分鐘左右(2000-3000轉(zhuǎn)/ 分)。仔細(xì)收集上清。 檢測(cè)細(xì)胞內(nèi)的成份時(shí),用PBS(PH7.2-7.4)稀釋細(xì)胞懸液,細(xì)胞濃度達(dá)到100萬(wàn)/ml左右。通過(guò)反復(fù)凍融,以 使細(xì)胞破壞并 放出細(xì)胞內(nèi)成份。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。保存過(guò)程中如有沉淀形成,應(yīng)再次離心。
5.組織標(biāo)本:切割標(biāo)本后,稱取重量。加入一定量的PBS,PH7.4。用液氮迅速冷凍保存?zhèn)溆?。?biāo)本融化后仍然保持2-8℃的溫度。加入一定量的PBS(PH7.4),用手工或勻漿器將標(biāo)本勻漿充分。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。分裝后一份待檢測(cè),其余冷凍備用。
PyroMark Q24 User Manual 9019715 qiagen
Mixer module w. top plate, PyroQ96ID 9019648 qiagen
Sensor, solenoid, EZ1xl 9019643 qiagen
Cable harness, x axis, C34 9019589 qiagen
PCB, mainboard, QGY 9019587 qiagen
End cap, Y-arm, Cxx 9019582 qiagen
Fuse, 2A 9019551 qiagen
Adapter, reagent tub plate, CX1 9019513 qiagen
TANK UPPER BUFFER ASSY GS 9019502 qiagen
miScript SYBR Green PCR Kit (200) 218073 qiagen
miScript Reverse Transcription Kit (50) 218061 qiagen
miRNeasy FFPE Kit (50) 217504 qiagen
Mother board ( micro board V 2.1 ) 9019441 qiagen
QuantiTect SYBR Green RT-PCR Kit (1000) 204245 qiagen
QuantiFast SYBR Green RT-PCR Kit (2000) 204156 qiagen
Rotor-Gene SYBR Green PCR Kit (400) 204074 qiagen
Hose, cooling blower, RG3 9019432 qiagen
Belt, toothed, source optics, RG3 9019423 qiagen
Gasket, cooling flap, RG3 9019394 qiagen
EPROM, IC main board, state F/W, RG3 9019385 qiagen
Gasket, lower chamber, RG6 9019367 qiagen
Flexitube siRNA 20 nmol W Modification 1027419 qiagen
FlexiPlate siRNA - 0.25 nmol 1027412 qiagen
Board, main, no microprocessor, RG6 9019323 qiagen
Mikrowin 9234699 qiagen
Cap - Vacuum bottle, PyroVW 9020439 qiagen
Coupling, CPC, screw, male 9234458 qiagen
Rack for Murex Reagents GE 14/15 (P1/3) 9233726 qiagen
Hinge kit - Robot, PyroQ96MD 9019199 qiagen
QIAsymphony Circulating NA Kit(48),cus,G 1074536 qiagen
Lid with LED, silver, RGQ 9019880 qiagen
PyroMark Computer (OPC), PyroQ96 9019839 qiagen
Service kit, pipettor 8 ch w/tool, Cx8 9019813 qiagen
Ipad 4 9022963 qiagen
Tray, buffer bottles, roter,BR8000 v1.00 9013833 qiagen
Switch - Vacuum, PyroQ96VW 9019662 qiagen
Interlock, IL1, EZ1xl 9019638 qiagen
Home flag, Y-axis, Cx2 9019614 qiagen
Motor, Y-axis, Cxx 9019568 qiagen
Pulley, motor, Al16T2.5/16-2, Cxx 9019557 qiagen
Motor, X-axis, Cxx 9019535 qiagen
miRNeasy Serum/Plasma Kit (50) 217184 qiagen
miRNeasy Micro Kit (50) 217084 qiagen
Kit, heater, FET, ICs, diode, cap, RG3 9019418 qiagen
Heater assy incl. element, sensors, RG3 9019408 qiagen
Damper, lid, RG3 9019399 qiagen
Rotor hub with shaft, RG6 9019355 qiagen
Impeller, heater fan, RGx 9019348 qiagen
FlexiPlate siRNA - 1 nmol 1027413 qiagen
AllStars Neg. siRNA AF 546 (20 nmol) 1027293 qiagen
Manual WinRufas 2.2 (CD) 9234695 qiagen
Tip rack tub, AS, Qsym 9020398 qiagen
Board, X-CPU, non-CE 9234657 qiagen
Display w. mount, PyroQ24 9019228 qiagen
Mainboard w. Lensblock & mixer, PyroQ24 9019227 qiagen
Power Supply - Instr., PyroQ24 9019222 qiagen
Cable - X robot, PyroQ96MD 9019207 qiagen
PG AIV-H5 RT-PCR Assay (48) LE Z 1066845 qiagen
FMDV RT-PCR REAGENT LE 1066852 qiagen
Buffer MW2 (87ml) 1060993 qiagen
HiSpeed Plasmid Mega EF Kit (5) 1054578 qiagen
HCV RT-PCR REAGENT VI 1061091 qiagen
HSV PCR REAGENT II 1061095 qiagen
HCMV Quantification Standards 1061100 qiagen
TB PCR REAGENT I 1061102 qiagen
MP Quantification Standards 1061106 qiagen
Security Key, LC Policy Manager, encoded 9016732 qiagen
Lid, label BC1, RG3 9019887 qiagen
Cover, main, label CoResearch, RG3 9019884 qiagen
Optical source and detector assy , RG3 9019883 qiagen
1061054 HBV PCR REAGENT I
1061069 UU PCR REAGENT II
1061079 NG PCR REAGENT II
9019886 Lid, label 4CH, RG3
9019875 Cover, main, silver, RGQ
9019836 IPC Hard Drive PCA6751, PyroQ96ID
9019769 Valve, shutoff, V2, QX
9019756 Valve, solenoid, V2, QX
9019734 Sensor, Z, with cable, V2, QX
9019728 Board, motor control, Y, V2, QX
9013832 Reagent Holder, 1-bottle Holder for acmodating 1 bottle (5 ml). For use with QIAGEN Protease on the BioRobot MDx worktable
9013824 BGR Tischplatte P3041 Robotec(SN602)
9013719 RS 232 Servicecabel
9013506 96-well Thermostat Base Plate1.02
9013484 Cooling and heating system, BR3000
9013442 Centrifuge, strip adaptor set
9013434 Maintenance kit, annual, BR9600 (A)
9019723 Transport lock, EZ1xl
9019656 Hose w. quick lock socket, PyroQ96VW
9019581 Pulley, Idler Y-axis, Cxx
9019554 Rail, Z-axis 138 mm, Cxx
9019552 Rail, X-axis 444 mm, CQx
218060 miScript Reverse Transcription Kit (10)
216513 FastLane Cell Multiplex Kit (200)
204254 QuantiFast Probe PCR Kit (400)
9019440 Solid State Relay
9019433 Transformer 2x115VAC O/P 2x11VAC
9019431 Kit, lock ring 36W incl. lockknob, RG3
9019402 Transformer, heater, RG3
1027321 Mm/Hs_MAPK1 control siRNA (20 nmol)
9019329 Serial connector USB, internal cable, RG
9019238 Heating block, PyroQ24
9020478 Heating plate, GSC
1026965 PCR Buffer,10 x (1,2/2) without MgCl2
9233466 Coupling, CPC, inline, male, 6mm
9233452 Coupling, X motor, BR8000
9019217 Lead screw - Y robot, PyroQ96MD
1017635 Protease Solvent 6x (10,2/15), Azid,KG
1066846 AIV-H5 RT-PCR REAGENT LE
1054250 Effectene Transfection Reag. Kit (0.3ml)
1061051 HBV PCR REAGENT II
9019890 Foam insert, rotor securing, QIAC
9019861 Rotor Locking Nut Set
9019821 IQ/OQ document, QS
9019768 Sensor, interlock2, with cable, QX
9019765 Belt, Y, V2, QX
9019727 Board, transport conversion, V2, QX
9013523 Underlay, vaccum manifold, v2.01
9013447 Control unit Y/Z, 4 channel
9013431 Maintenance kit, semi annual, BR9600 (E)
9238807 Round carrier for 12 Tubes 15ml
9019715 PyroMark Q24 User Manual
9019648 Mixer module w. top plate, PyroQ96ID
9019643 Sensor, solenoid, EZ1xl
9019589 Cable harness, x axis, C34
9019587 PCB, mainboard, QGY
9019582 End cap, Y-arm, Cxx
9019551 Fuse, 2A
9019513 Adapter, reagent tub plate, CX1
9019502 TANK UPPER BUFFER ASSY GS
218073 miScript SYBR Green PCR Kit (200)
218061 miScript Reverse Transcription Kit (50)
217504 miRNeasy FFPE Kit (50)
9019441 Mother board ( micro board V 2.1 )
204245 QuantiTect SYBR Green RT-PCR Kit (1000) For 1000 x 50 ul reactions: 25 ml QuantiTect SYBR Green RT-PCR Master Mix (providing a final concentration of 2.5 mM MgCl2), 0.5 ml QuantiTect RT Mix, 20 ml RNase-free water
204156 QuantiFast SYBR Green RT-PCR Kit (2000)
204074 Rotor-Gene SYBR Green PCR Kit (400) The Rotor-Gene SYBR Green PCR Kit is dedicated for use with the Rotor-Gene Q cycler, providing ultrafast, highly specific quantification in real-time PCR and two-step RT-PCR using SYBR Green I.
9019432 Hose, cooling blower, RG3
9019423 Belt, toothed, source optics, RG3
9019394 Gasket, cooling flap, RG3
9019385 EPROM, IC main board, state F/W, RG3
9019367 Gasket, lower chamber, RG6
1027419 Flexitube siRNA 20 nmol W Modification
1027412 FlexiPlate siRNA - 0.25 nmol
9019323 Board, main, no microprocessor, RG6
9234699 Mikrowin
9020439 Cap - Vacuum bottle, PyroVW
9234458 Coupling, CPC, screw, male
9233726 Rack for Murex Reagents GE 14/15 (P1/3)
9019199 Hinge kit - Robot, PyroQ96MD
1074536 QIAsymphony Circulating NA Kit(48),cus,G
9019880 Lid with LED, silver, RGQ
9019839 PyroMark Computer (OPC), PyroQ96
9019813 Service kit, pipettor 8 ch w/tool, Cx8
9022963 Ipad 4
9013833 Tray, buffer bottles, roter,BR8000 v1.00
9019662 Switch - Vacuum, PyroQ96VW
9019638 Interlock, IL1, EZ1xl
9019614 Home flag, Y-axis, Cx2