艱難梭菌毒素A(ClostridiumdifficiletoxinA)檢測試劑盒齊一生物長期經(jīng)營ELISA試劑盒(免費(fèi)代測)及抗體,分子學(xué)生物試劑盒,金標(biāo)法檢測試劑盒,比色法檢測試劑盒,細(xì)胞菌株,培養(yǎng)基,細(xì)胞因子,生化試劑,耗材等生物試劑產(chǎn)品.誠信經(jīng)營,價(jià)格實(shí)惠,服務(wù)周到,質(zhì)量保障!提供各種實(shí)驗(yàn)代測服務(wù).歡迎新老客戶!齊一生物科技(上海)有限公司,專業(yè)生產(chǎn)研發(fā)生化科研試劑全國
艱難梭菌毒素A(ClostridiumdifficiletoxinA)檢測試劑盒操作步驟
實(shí)驗(yàn)開始前,請?zhí)崆芭渲煤盟性噭噭┗驑悠废♂寱r(shí),均需混勻,混勻時(shí)盡量避免起泡。每次檢測都應(yīng)該做標(biāo)準(zhǔn)曲線。如樣品濃度過高時(shí),用樣品稀釋液進(jìn)行稀釋,以使樣品符合試劑盒的檢測范圍。
1. 加樣:分別設(shè)空白孔、標(biāo)準(zhǔn)孔、待測樣品孔??瞻卓准訕悠废♂屢?00μl,余孔分別加標(biāo)準(zhǔn)品或待測樣品100μl,注意不要有氣泡,加樣將樣品加于酶標(biāo)板孔底部,盡量不觸及孔壁,輕輕晃動(dòng)混勻,酶標(biāo)板加上蓋或覆膜,37℃反應(yīng)120分鐘。
為保證實(shí)驗(yàn)結(jié)果有效性,每次實(shí)驗(yàn)請使用新的標(biāo)準(zhǔn)品溶液。
2. 棄去液體,甩干,不用洗滌。每孔加生物素標(biāo)記抗體工作液 100μl(取1μl生物素標(biāo)記抗體加99μl生物素標(biāo)記抗體稀釋液的比例配制,輕輕混勻,在使用前一小時(shí)內(nèi)配制),37℃,60分鐘。
3. 溫育60分鐘后,棄去孔內(nèi)液體,甩干,洗板3次,每次浸泡1-2分鐘,350μl/每孔,甩干。
4. 每孔加辣根過氧化物酶標(biāo)記親和素工作液(同生物素標(biāo)記抗體工作液) 100μl,37℃,60分鐘。
5. 溫育60分鐘后,棄去孔內(nèi)液體,甩干,洗板5次,每次浸泡1-2分鐘,350μl/每孔,甩干。
6. 依序每孔加底物溶液90μl,37℃避光顯色(30分鐘內(nèi),此時(shí)肉眼可見標(biāo)準(zhǔn)品的前3-4孔有明顯的梯度藍(lán)色,后3-4孔梯度不明顯,即可終止)。
7. 依序每孔加終止溶液50μl,終止反應(yīng)(此時(shí)藍(lán)色立轉(zhuǎn)黃色)。終止液的加入順序應(yīng)盡量與底物液的加入順序相同。為了保證實(shí)驗(yàn)結(jié)果的準(zhǔn)確性,底物反應(yīng)時(shí)間到后應(yīng)盡快加入終止液。
8. 用酶聯(lián)儀在450nm波長依序測量各孔的光密度(OD值)。 在加終止液后15分鐘以內(nèi)進(jìn)行檢測。
Motor Cap Repair, Eppendorf, AP196/98 9240548 qiagen
I/O PCB, Main Board, AP196/98 9240515 qiagen
PM Kit, AP196 9240503 qiagen
BR M48, PM Service 9239958 qiagen
Foot, plete, v2.0 (4) 9239853 qiagen
BR Universal, Full Agreement 9239572 qiagen
Assy,Kit,Retrofit,HoriCompressorMnt 9239563 qiagen
LED UV (325), RGQ 9020033 qiagen
L 26_7 Firmware, DML2 9020023 qiagen
PCB, UV light assembly, Cxx 9019975 qiagen
Basic Cover Agreement Plato7, 1yr 9238858 qiagen
User Manual, QIAxcel FA 01/08 (WW) 9241233 qiagen
ZLV, Handscanner, Qsym 9241227 qiagen
Motor, Z, QX 9241180 qiagen
Loading guide, 52 well sharkstooth, GSC 9019473 qiagen
Switch, micro, HS 9019462 qiagen
Fans (2), 5V, 30mm, HS 9019457 qiagen
Cable USB 9237172 qiagen
Board, YZ-CPU, BR8000 9233169 qiagen
Bezel LiquiChip 9237163 qiagen
Shipping Plug XYP 9237162 qiagen
#Twister I, Full Agreement, no PM 9237135 qiagen
Protocol Dev./Opt., on-site (5-days) 9237109 qiagen
Upgrade, BR plus 2 ext. devices 9237102 qiagen
Card, x-main, peristaltic XPUPG 9232974 qiagen
YZ 96 CPU Board/Driver Unit 9232972 qiagen
Springcontact, RoboVac, BR8000 9232947 qiagen
Storage Platform TW I, deep well 9236408 qiagen
Board, PCON, auto vac, BR8000 9232918 qiagen
Green LSR 9236334 qiagen
Fan, LiquiChip 9236324 qiagen
Locator Plate, 96 well,pos 2,BR RP 9236151 qiagen
Rack for reagents, Universal (zig-zag) 9230820 qiagen
Predilution-Rack 320x2ml Rose-80 9230718 qiagen
Cable,Interface,flat,(Syr.)-Incub.-Read. 9230365 qiagen
Safety lock, waste drawer, v1, Qsym 9021284 qiagen
Rubber seal, centrifuge motor 9238611 qiagen
Tubing, OLF pump head, RapidPlate 9238477 qiagen
Tray for Reaction 9238245 qiagen
Upgrade App., DirectPrep 96 9238191 qiagen
EZ1, Full Agreement 9237721 qiagen
Bimba Cylinder, Latch Lock, AP98 9240676 qiagen
Nut, Flangeless,1/4-28, Blue, AP 9240668 qiagen
Power Supply, 48VDC 4.0 AMP, AP96 9240632 qiagen
Pressure Gauge, Digital, AP196/98 9240605 qiagen
Sensor, Sick, Waste Btl. Present, AP 9240602 qiagen
Control Pad, Eppendorf, AP196/98 9240561 qiagen
Temp sensor, Bowl, Eppendorf, AP196/98 9240558 qiagen
Right Angle Con., SiNug, Motor, AP96/196 9240540 qiagen
Motor Assy, SiNug, 23, Pump, AP96/196 9240535 qiagen
Cable, Dual, USB transition, AP196/98 9240517 qiagen
Holder, click'n go cassettes, HSPS 9240195 qiagen
Re-Installation, BR MDx 9240101 qiagen
Pneumatic Cylinder, v2.0 9239856 qiagen
CRL-1740 Lncap(前列腺癌細(xì)胞) ATCC 株
CRL-1435 PC-3(前列腺癌細(xì)胞) ATCC 株
CRL-1582 MOLT-4(急性淋巴母細(xì)胞白血病細(xì)胞) ATCC 株
HTB-9 5637(膀胱癌細(xì)胞) ATCC 株
CCL-86 Raji(Burkitt's 淋巴瘤細(xì)胞) ATCC 株
HTB-3 SCaBER(膀胱鱗癌細(xì)胞) ATCC 株
CRL-1432 NAMALWA(Burkitt's 淋巴瘤細(xì)胞) ATCC 株
HTB-4 T24(膀胱移行細(xì)胞癌細(xì)胞) ATCC 株
TIB-161 HuT78(T淋巴細(xì)胞白血病細(xì)胞) ATCC 株
CCL-105 SW-13(腎上腺皮質(zhì)腺癌細(xì)胞 ) ATCC 株
CCL-105 SW-13(腎上腺皮質(zhì)腺癌細(xì)胞 ) ATCC 株
Dami(巨核細(xì)胞白血病細(xì)胞) ATCC 株
// OS-RC-2(腎癌細(xì)胞) ATCC 株
CRL-2021 MEG-01 ATCC 株
CRL-1932 786-O [786-0](腎透明細(xì)胞腺癌細(xì)胞) ATCC 株
CRL-1932 786-O [786-0](腎透明細(xì)胞腺癌細(xì)胞) ATCC 株
CCL-243 K-562(慢性髓原白血病細(xì)胞) ATCC 株
CCL-243 K-562(慢性髓原白血病細(xì)胞) ATCC 株
// Hce-8693(盲腸腺癌細(xì)胞(未分化)) ATCC 株
CCL-137 HEL299(紅白細(xì)胞白血病細(xì)胞) ATCC 株
// CW-2(結(jié)腸腺癌細(xì)胞) ATCC 株
CRL-1619 A-375 [A375](惡性黑色素瘤細(xì)胞) ATCC 株
// COLO-320(結(jié)腸腺癌細(xì)胞) ATCC 株
CRL-1620 A172(膠質(zhì)母細(xì)胞瘤細(xì)胞) ATCC 株
CCL-228 SW480 [SW-480](結(jié)腸癌細(xì)胞) ATCC 株
// SHG-44(膠質(zhì)瘤細(xì)胞) ATCC 株
HTB-37 Caco-2(結(jié)腸腺癌細(xì)胞) ATCC 株
HTB-37 Caco-2(結(jié)腸腺癌細(xì)胞) ATCC 株
// U251(膠質(zhì)瘤細(xì)胞) ATCC 株
CL-188 LS 174T(結(jié)腸腺癌細(xì)胞) ATCC 株
HTB-10 SK-N-MC(神經(jīng)上皮瘤細(xì)胞) ATCC 株
HTB-10 SK-N-MC(神經(jīng)上皮瘤細(xì)胞) ATCC 株
CRL-1687 BxPC-3(原位胰腺腺癌細(xì)胞) ATCC 株
CRL-1687 BxPC-3(原位胰腺腺癌細(xì)胞) ATCC 株
// KP-N-NS(腎上腺神經(jīng)母細(xì)胞瘤細(xì)胞(腦轉(zhuǎn)移)) ATCC 株
CRL-1682 AsPC-1(轉(zhuǎn)移胰腺腺癌細(xì)胞) ATCC 株
CRL-1682 AsPC-1(轉(zhuǎn)移胰腺腺癌細(xì)胞) ATCC 株
HTB-96 U-2 OS(骨肉瘤細(xì)胞) ATCC 株
HTB-96 U-2 OS(骨肉瘤細(xì)胞) ATCC 株
// GBC-SD(膽囊癌細(xì)胞) ATCC 株
CCL-136 RD(惡性胚胎橫紋肌瘤細(xì)胞) ATCC 株
CCL-136 RD(惡性胚胎橫紋肌瘤細(xì)胞) ATCC 株
// HCCC-9810(膽管細(xì)胞型肝癌細(xì)胞) ATCC 株
CRL-1598 A-673(橫紋肌瘤細(xì)胞) ATCC 株
// SMMC-7721(肝癌細(xì)胞) ATCC 株
// SMC-1(胸膜細(xì)胞瘤) ATCC 株
// QGY-7703(肝癌細(xì)胞) ATCC 株
HTB-171 NCI-H446 [H446](小細(xì)胞肺癌細(xì)胞) ATCC 株
// QGY-7701(肝癌細(xì)胞) ATCC 株
// BEL-7405(肝癌細(xì)胞) ATCC 株
HTB-177 NCI-H460 [H460](大細(xì)胞肺癌細(xì)胞0 ATCC 株
HTB-177 NCI-H460 [H460](大細(xì)胞肺癌細(xì)胞0 ATCC 株
// BEL-7404(肝癌細(xì)胞) ATCC 株
// BEL-7402(肝癌細(xì)胞) ATCC 株
// QG-56(肺扁平上皮癌細(xì)胞) ATCC 株
// SPC-A-1(肺腺癌細(xì)胞) ATCC 株
// HGC-27(胃癌細(xì)胞(未分化)) ATCC 株
// LTEP-a-2(肺腺癌細(xì)胞) ATCC 株
CRL-1739 AGS(胃腺癌細(xì)胞) ATCC 株
// MGC80-3(胃癌細(xì)胞) ATCC 株
// 95-D(高轉(zhuǎn)移肺癌細(xì)胞) ATCC 株
// BGC-823(胃腺癌細(xì)胞(低分化)) ATCC 株