百日咳IgG(PT-IgG)檢測試劑盒(血清.血漿.組織)齊一生物針對中客戶推出自主品牌 ELISA KIT(96T/48T),為了保證客戶用貨質(zhì)量,我公司采用純進(jìn)口原料現(xiàn)包被生產(chǎn),質(zhì)量穩(wěn)定,性價比高!!提供代測服務(wù)!全國:
百日咳IgG(PT-IgG)檢測試劑盒(血清.血漿.組織)此IBL試劑盒能用于小鼠血清,EDTA血漿,細(xì)胞上清中產(chǎn)品定量檢測試劑盒成分
1預(yù)包被板:抗小鼠白介素-6兔子IgG,親合純化96T
2酶標(biāo)記抗體:(30倍濃縮)HRP標(biāo)記抗小鼠白介素-6兔子IgG,親合純化0.4mLx1
3標(biāo)準(zhǔn)品:重組小鼠白介素-60.5mLx2
4EIA緩沖液:含1%BSA,0.05%吐溫20BPS30mLx1
5標(biāo)記抗體稀釋液:含1%BSA,0.05%吐溫20BPS12mLx1
6顯色劑:TMB底物液15mLx1
7終止液:1N硫酸12mLx1
8濃縮洗滌液:(40倍濃縮)含1%BSA,0.05%吐溫20BPS50mLx1操作說明1實驗所需器材(但試劑盒沒有提供)酶標(biāo)儀(450nm)微移液管及其吸嘴量筒及燒杯去離子水冰箱(4°C)坐標(biāo)紙(log/log)吸水紙試管(用于標(biāo)準(zhǔn)品稀釋)溫育箱(37°C±1°C)洗瓶(用于洗板)一次性試劑管(用于濃縮酶標(biāo)記抗體和顯色劑)
操作方法
雙抗體夾心法
1.包被:用0.05MPH9.牰碳酸鹽包被緩沖液將抗體稀釋蛋白質(zhì)含量為1~10μg/ml.在每個聚苯乙烯板的反應(yīng)孔中加0.1ml,4℃隔夜.次日,棄去孔內(nèi)溶液,用洗滌緩沖液洗3次,每次3分鐘.(簡稱洗滌,下同).
2. 加樣:加一定稀釋的待檢樣品0.1ml于上述已包被之反應(yīng)孔中,置37℃孵育1小時.然后洗滌.(同時做空白孔,陰性對照孔及陽性對照孔).
3. 加酶標(biāo)抗體:于各反應(yīng)孔中,加入新鮮稀釋的酶標(biāo)抗體(經(jīng)滴定后的稀釋度)0.1ml.37℃孵育0.5~1小時,洗滌.
4. 加底物液顯色:于各反應(yīng)孔中加入臨時配制的TMB底物溶液0.1ml,37℃10~30分鐘.
5. 終止反應(yīng):于各反應(yīng)孔中加入2M硫酸0.05ml.
6. 結(jié)果判定:可于白色背景上,直接用肉眼觀察結(jié)果:反應(yīng)孔內(nèi)顏色越深,陽性程度越強(qiáng),陰性反應(yīng)為無色或極淺,依據(jù)所呈顏色的深淺,以“+”、“-”號表示.也可測OD值:在ELISA檢測儀上,于450nm(若以ABTS顯色,則410nm)處,以空白對照孔調(diào)零后測各孔OD值,若大于規(guī)定的陰性對照OD值的2.1倍,即為陽性.
Anti-HHV8/ORF50(Human herpesvirus 8 type P) 人類皰疹病毒8抗體
Anti-HHV8/ORF50(Human herpesvirus 8 type P) 人類皰疹病毒8抗體
Anti-HHV8/ORF50(Human herpesvirus 8 type P) 人類皰疹病毒8抗體
Anti-HHV8/ORF K2(Human herpesvirus 8 type P) 人類皰疹病毒8抗體
Anti-HIF-1α (Hypoxia-inducible factor -1α) 缺氧誘導(dǎo)因子1α 抗體
Anti-Histone H3(Family 3A、HISTIH3I protein、Histih 3A protein) 抗組蛋白抗體(AHA)
Anti-Histone H3-like protein 抗組蛋白H3樣抗體(N端抗體)
Anti-Histone H3-like protein 抗組蛋白H3樣抗體(C端抗體)
Anti-Histone H3-like protein 抗組蛋白H3樣抗體(N端抗體)
Anti-HisX6/6His/His tag 聚組氨酸抗體/抗His tag標(biāo)簽抗體
anti-DYKDDDDK Tag (NT)/Flag-Tag polyclonal antibody 抗標(biāo)簽DYKDDDDK Tag抗體(N端)
anti-DYKDDDDK Tag (CT)/Flag-Tag polyclonal antibody 抗標(biāo)簽DYKDDDDK Tag抗體(C端)
Anti-HIV gp120 艾滋病病毒抗體
Anti-HIV gp41 艾滋病病毒抗體
Anti-HIV-1(human immunodeficiency virus type 1) 艾滋病病毒-1/人類免疫缺陷病毒1型抗體
Anti-HIV-1(human immunodeficiency virus type 1)Anti-Anti-Anti-Anti-HIV-1(human immunodeficiency virus type 1) 艾滋病病毒-1/人類免疫缺陷病毒1型抗體
Anti-HLA-DR (human leukoyte antigen DR) HLA-DR抗原抗體
Anti-HLA-G (Human Leukocyte Antigen-G) 人類白細(xì)胞抗原G/組織相容性白細(xì)胞抗原G抗體
Anti-HLA-E (Human Leukocyte Antigen-E) 人類白細(xì)胞抗原E
Anti-HMGA1 (high mobility group AT-hook 1) 高遷移率族蛋白A1抗體
Anti-HMGA2 (high mobility group A2) 高遷移率族蛋白A2抗體
Anti-HMGB1 (High Mobility Group Box protein 1) 高遷移率族蛋白-1抗體
Anti-HO-1/HSP32 (Hemeo xygenase 1) 血紅素氧合酶-1/熱休克蛋白-32抗體
Anti-HO-2 (Heme oxygenase 2) 血紅素氧合酶-2抗體
Anti-HOXc8 同源盒基因的一種
Anti-HPV (Human papillomavirus) 人類乳頭狀瘤病毒抗體
Anti-H-ras 原癌基因H-ras抗體
Anti-HSL(hormone-sensitive lipase) 荷爾蒙敏感脂肪酸抗體
Anti-HSP-27(Heat Shock Protein 27) 熱休克蛋白-27抗體
Anti-HSP-60( Heat shock protein-60) 熱休克蛋白-60抗體
Anti-HSP-70 (Heat shock protein-70) 熱休克蛋白-70抗體
Anti-HSP-90α(Heat Shock Protein 90α ) 熱休克蛋白90α抗體
Anti-HSP-90α(Heat Shock Protein 90α ) 熱休克蛋白90α抗體
Anti-HSP-90β (heat sock protein-90β) 熱休克蛋白-90β 抗體
anti-HtrA2/Omi(mitochondrial intermembrane space) 絲氨酸蛋白酶/線粒體絲氨酸蛋白酶抗體
Anti-hTRT omerase catalytic subunit(TE) 端粒酶抗體(催化亞單位)
Anti-Human serumAnti-Human serum 兔抗人血清抗體抗血清
Anti-Hydroxyethyk starch/HES Anti-hydroxyethyk starch/HES 羥yi基淀粉抗體
Anti-Hypothetical protein (LOC55652) hypothetical protein抗體
anti-IAA(Indole-3-Acetic Acid) 吲哚-3-乙酸抗體/植物生長素抗體
anti-IAA(Indole-3-Acetic Acid) 吲哚-3-乙酸抗體/植物生長素單克隆抗體
anti-IAP-5(inhibitors of apoptosis protein-5) 凋亡抑制因子5抗體
Anti-IASPP(inhibitory member of the ASPP family) 腫瘤細(xì)胞凋亡ASPP家族的另一個成員抗體
Anti-ICAD(Inhibitor of Caspase-Activated Dnase ) Caspase 激活的脫氧核糖核酸酶抑制劑
Anti-IDE(Insulin degradation enzyme) 胰島素降解酶抗體
Mouse Anti-influenza A virus nucleoprotein Monoclonal(Flu-A) 鼠抗人禽流感A核蛋白單克隆抗體
Mouse Anti-influenza B virus nucleoprotein Monoclonal(Flu-B) 鼠抗人禽流感B核蛋白單克隆抗體
Anti-IFABP(Intestinal Fatty acid binding protein) 小腸型脂肪酸結(jié)合蛋白抗體
Anti-IFITM1 (interferon induced transmembrane protein 1) 干擾素誘導(dǎo)跨膜蛋白1抗體
Anti-IFN-β (Interferon β)Anti-human 干擾素-β抗體(抗人)
Anti-IFN-β (Interferon β)Anti-mouse,rat 干擾素-β抗體(抗大鼠、小鼠)
Anti-IFN-γ(Mouse Anti-Human Interferon γ Monoclonal Antibody) 鼠抗人干擾素-γ/IFN-gamma單克隆抗體
Anti-IFN-γ(Interferon γ)Anti-Human 干擾素-γ抗體(抗人)
Anti-IFN-γ(Interferon γ)Anti-mouse、rat 干擾素-γ抗體 (抗大、小鼠)
31432 Pierce(R) Peroxidase Conjugated Goat Anti-Mouse IgG (H+L) 1.5 ML
31433 Pierce(R) Peroxidase Conjugat ed Rabbit Anti-Goat IgG (Fc) 1.5 ML
31436 Pierce(R) Peroxidase Conjugated Goat Anti-Mouse IgG F(ab')2 2 ML
31437 Pierce(R) Peroxidase Conjugated Goat Anti-Mouse IgG (Fc) 2.0 ML
31438 Pierce(R) Peroxidase Conjugated F(ab')2 Fragment Goat Anit- 0.5 ML
31439 Pierce(R) Peroxidase Conjugated Goat Anti-Mouse IgG (Fc) (mi 1.5 ML
31440 Pierce(R) Peroxidase Conjugated Goat Anti-Mouse IgMu, 2 ML 2 ML
31444 Pierce(R) Peroxidase Conjugated Goat Anti-Mouse IgG+IgM (H+ 2 ML
31446 Pierce(R) Peroxidase Conjugated Goat Anti-Mouse IgG+IgM (H+L 1.5 ML
31448 Pierce(R) Peroxidase Conjugated F(ab')2 Fragment Goat Anti- 0.5 ML
31450 Pierce(R) Peroxidase Conjugated Rabbit Anti-Mouse IgG (H+L) 1.5 ML
31451 Pierce(R) Peroxidase Conjugated Rabbit Anti-Mouse IgG {F(ab 1.5 ML
31452 Pierce(R) Peroxidase Conjugated Rabbit Anti-Mouse IgG (H+L) 1 ML
31453 Pierce(R) Mouse Transferrin Peroxidase Conjugated, 1 mg 1 MG
31455 Pierce(R) Peroxidase Conjugated Rabbit Anti-Mouse IgG (Fc) 1.5 ML
31456 Pierce(R) Peroxidase Conjugated Rabbit Anti-Mouse IgM (u) 1.5 ML
31457 Pierce(R) Peroxidase Conjugated Rabbit Anti-Mouse IgG + IgM 1.5 ML
31458 Pierce(R) Peroxidase Conj. Donkey Anti-Rabbit IgG(H+L) 0.5 ML
31460 Pierce(R) Goat Anti-Rabbit IgG, (H&L), Peroxidase Conjugated 2 ML
31461 Pierce(R) Peroxidase Conjugat ed Goat Anti-Rabbit IgG {F(ab' 2.0 ML
31462 Pierce(R) Peroxidase Conjugated Goat Anti-Rabbit IgG (H+L) 1.5 ML
31463 Pierce(R) Peroxidase Conjugat ed Goat Anti-Rabbit IgG (Fc) 2.0 ML
31464 Pierce(R) Peroxidase Conjugated Mouse Anti-Rabbit IgG (H+L) 1.0 ML
31465 ImmunoPure Human IgG, Whole Molecule-Peroxidase Conjugated, 1 MG
31466 ImmunoPure Peroxidase Conjugated Goat Anti-Rabbit 0.2 ML
31470 Pierce(R) Goat Anti-Rat IgG, (H&L), Peroxidase Conjugated, 2 ML
31471 ImmunoPure Peroxidase Conjugated Goat Anti-Rat 0.2 MG
31474 Pierce(R) Peroxidase Conjugated Goat Anti-Rat IgG F(ab')2, 2 ML
31475 Pierce(R) Peroxidase Conjugated Goat Anti-Rat IgG (Fc) 2.0 ML
31476 Pierce(R) Peroxidase Conjugated Goat Anti-Rat IgMu, 2 ML 2 ML
31480 Pierce(R) Peroxidase Conjugated Rabbit Anti-Sheep IgG (H+L), 1.5 ML
31482 ImmunoPure (R) Peroxidase Conjugated Goat Anti-Human IgG 2 ML
31485 EZ-Link Maleimide Activated Horseradish Peroxidase 5 mg
31487 EZ-Link Plus Activated Peroxidase 1 mg
31488 EZ-Link Plus Activated Peroxidase 5 X 1 mg
31489 EZ-Link Plus Activated Peroxidase Kit Kit
31490 Horseradish Peroxidase (HRP) 10 mg
31491 Horseradish Peroxidase 100 mg
31494 EZ-Link Maleimide Activated Horseradish Peroxidase Kit Kit
31497 EZ-Link Activated Peroxidase Antibody Labeling Kit Kit
31499 Rebinant Protein G, Peroxidase Conjμgated 0.5 mg
31501 Pierce(R) Fluorescein Conjugated Rabbit Anti-Chicken IgY (H 1.5 MG
31503 Pierce Peroxidase Conjμgate Stabilizer 25 mL
31505 ImmunoPure Mouse IgG, Whole Molecule- Fluorescein Conjugate 1 MG
31509 Pierce(R) Fluorescein Conjugated Rabbit Anti-Goat IgG, 1.5 MG
31512 Pierce(R) Fluorescein Conjugated Mouse Anti-Goat IgG (H&L)mi 1.0 MG
31529 Pierce(R) Fluorescein Conjugated Goat Anti-Human IgG (H+L), 2 MG
31531 Pierce(R) Fluorescein Conjugated Goat Anti-Human IgG (H+L), 1.5 MG
31533 Pierce(R) Fluorescein Conjugated Rabbit Anti-Goat IgG (Fc) 1.5 MG
31535 Pierce(R) Fluorescein Conjugated Rabbit Anti-Human IgG (Fc) 1.5 MG
31539 Pierce(R) Fluorescein Conjugated F(ab')2 Fragment Goat Anti 1 MG
31541 ImmunoPure Fluorescein Conjugated Goat Anti-Mouse IgG (H+L)( 1.5 MG
31543 Pierce(R) Fluorescein Conjuga ted Goat Anti-Mouse IgG F(ab') 2 MG
31547 Pierce(R) Fluorescein Conjuga ted Goat Anti-Mouse IgG (Fc) 2.0 MG
31553 Pierce(R) Fluorescein Conjugated Rabbit Anti-Goat IgG 1.5 MG
31555 Pierce(R) Fluorescein Conjugated Rabbit Anti-Mouse IgG (Fc) 1.5 MG
31557 Pierce(R) Fluorescein Conjugated Rabbit Anti-Mouse IgM (u) 1.5 MG
31559 Pierce(R) Fluorescein Conjugated Rabbit Anti-Mouse IgG 1.5 MG
31561 Pierce(R) Fluorescein Conjugated Rabbit Anti-Mouse IgG (H+L 1.5 MG
31565 Pierce(R) Fluorescein Conjugated F(ab')2 Fragment Goat Anti 1 MG
31568 Pierce(R) Fluorescein Conjugated Donkey Anti-Rabbit IgG (H+ 0.5 MG
31569 Pierce(R) Fluorescein Conjugated Goat Anti-Mouse IgG (H+L), 2 MG
31573 Pierce(R) Fluorescein Conjugated Goat Anti-Rabbit IgG 2.0 MG
31575 Pierce(R) Fluorescein Conjugated Goat Anti-Human IgM Fc5u, 2 MG
31577 Pierce(R) Fluorescein Conjugated Goat Anti-Human IgAa, 2 mg 2 MG
31579 Pierce(R) Fluorescein Conjugated F(ab')2 Fragment Goat Anti 1 MG
31583 Pierce(R) Fluorescein Conjugated Goat Anti-Rabbit IgG (H+L) 1.5 MG
31584 Pierce(R) Fluorescein Conjugated Mouse Anti-Rabbit IgG (H+L 1 MG
31587 ImmunoPure Rabbit Anti-Syrian Hamster IgG, (H+L) Fluorescein 1.5 MG