小鼠碳酸酐酶（CA）檢測(cè)試劑盒英文名稱(chēng)Mouse carbonic anhydrase,CA ELISA Kit價(jià)格：全國(guó)：,到我司741653262,齊一生物科技（上海）有限公司提供大量品牌進(jìn)口原裝,分裝,以及穩(wěn)定性強(qiáng)的國(guó)產(chǎn)ELISA試劑盒,性?xún)r(jià)比*.
小鼠碳酸酐酶（CA）檢測(cè)試劑盒ELISA試劑盒操作步驟
ELISA試劑盒使用前,將所有試劑充分混勻.不要使液體產(chǎn)生大量的泡沫,以免加樣時(shí)加入大量的氣泡,產(chǎn)生加樣上的誤差.
根據(jù)待測(cè)樣品數(shù)量加上標(biāo)準(zhǔn)品的數(shù)量決定所需的板條數(shù).每個(gè)標(biāo)準(zhǔn)品和空白孔建議做復(fù)孔.每個(gè)樣品根據(jù)自己的數(shù)量來(lái)定,能使用復(fù)孔的盡量做復(fù)孔.標(biāo)本用標(biāo)本稀釋液1：1稀釋后加入50ul于反應(yīng)孔內(nèi).
加入稀釋好后的標(biāo)準(zhǔn)品50ul于反應(yīng)孔、加入待測(cè)樣品50ul于反應(yīng)孔內(nèi).立即加入50ul的生物素標(biāo)記的抗體.蓋上膜板,輕輕振蕩混勻,37℃溫育1小時(shí).
甩去孔內(nèi)液體,每孔加滿(mǎn)洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干.重復(fù)此操作3次.如果用洗板機(jī)洗滌,洗滌次數(shù)增加一次.
每孔加入80ul的親和鏈酶素-HRP,輕輕振蕩混勻,37℃溫育30分鐘.
甩去孔內(nèi)液體,每孔加滿(mǎn)洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干.重復(fù)此操作3次.如果用洗板機(jī)洗滌,洗滌次數(shù)增加一次.
每孔加入底物A、B各50ul,輕輕振蕩混勻,37℃溫育10分鐘.避免光照.
取出酶標(biāo)板,迅速加入50ul終止液,加入終止液后應(yīng)立即測(cè)定結(jié)果.
在450nm波長(zhǎng)處測(cè)定各孔的OD值.
GPR173 Antibody WB ELISA
GPR173 Antibody WB ELISA
GPR174 Antibody WB ELISA
GPR176 Antibody WB ELISA
GPR18 Antibody WB ELISA
GPR180 Antibody WB IHC ELISA
GPR25 Antibody WB ELISA
GPR27 Antibody WB ELISA
GPR37 Antibody WB ELISA
GPR62 Antibody WB ELISA
GPR83 Antibody WB ELISA
GPRC5A Antibody WB ELISA
GPRC5D Antibody WB ELISA
GPRC6A Antibody WB ELISA
GR Antibody WB ELISA
GRAP2 Antibody WB ELISA
GRB2 Antibody WB ELISA
GRID1 Antibody WB IHC ELISA
GRIN2B Antibody WB ELISA
GRK1 Antibody WB IHC ELISA
GRK3 Antibody WB ELISA
GRM5 Antibody WB ELISA
GRM7 Antibody WB ELISA
GRTP1 Antibody WB ELISA
GSDMC Antibody WB ELISA
GUSB Antibody WB ELISA
GUSBL1 Antibody WB ELISA
HBAP1 Antibody WB ELISA
HBP1 Antibody WB ELISA
HCN2 Antibody WB ELISA
HCRTR1 Antibody WB ELISA
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卵黃氯化鈉瓊脂
卵磷脂吐溫-80營(yíng)養(yǎng)瓊脂
卵磷脂吐溫-80營(yíng)養(yǎng)瓊脂
馬鈴薯葡萄糖瓊脂
馬鈴薯葡萄糖瓊脂
馬鈴薯葡萄糖瓊脂
麥康凱瓊脂
麥康凱瓊脂
麥康凱瓊脂
麥康凱瓊脂
玫瑰紅鈉(虎紅)瓊脂培養(yǎng)基
玫瑰紅鈉(虎紅)瓊脂培養(yǎng)基
孟加拉紅瓊脂
平板計(jì)數(shù)瓊脂
平板計(jì)數(shù)瓊脂
平板菌落計(jì)數(shù)瓊脂
葡萄糖瓊脂
慶大霉素瓊脂
瓊脂，顆粒狀
瓊脂粉
瓊脂粉
瓊脂粉
瓊脂條
瓊脂Noble
瓊脂VRB
肉湯瓊脂培養(yǎng)基
88275 Pierce High Capacity Endotoxin Removal Spin Columns, 0.5mL 25 X 0.5 mL
88276 Pierce High Capacity Endotoxin Removal Spin Columns, 1mL 5 X 1 mL
88277 Pierce High Capacity Endotoxin Removal Spin Columns, 1mL 25 X 1 mL
88279 Pierce Mouse Embryonic Fibroblast Isolation Kit KIT
88280 Pierce Primary Neuron Isolation Kit DAFA KIT
88281 Pierce Primary Cardiomyocyte Isolation Kit DAFA KIT
88282 Pierce LAL Chromogenic Endotoxin Quantitation Kit Kit
88283 Neuronal Culture Medium 500mL
88284 Hanks’ Balanced Salt Solution (HBSS, without Ca2+, Mg2+) 500mL
88285 Neuronal Isolation Enzyme (with papain), lyophilized 1 VIAL
88286 Neuronal Culture Media Supplement (50X) 10mL
88287 DMEM for Primary Cell Culture 500mL
88288 Cardiomyocyte Isolation Enzyme 1 (with papain), lyophilized 1 vial
88289 Cardiomyocyte Isolation Enzyme 2 (with thermolysin), frozen liquid 100μL
88290 Mouse Embryonic Fibroblast Isolation Enzyme (with papain), lyophilized 1EA
88291 D-Luciferin, Monosodium Salt 100mg
88292 D-Luciferin, Monosodium Salt 1g
88293 D-Luciferin, Monopotassium Salt 100mg
88294 D-Luciferin, Monopotassium Salt 1g
88300 Fe-NTA Phosphopeptide Enrichment Kit 30 PACK
88301 Pierce TiO2 Phosphopeptide Enrichment and Clean-up Kit 24 TIPS/Kit
88302 Pierce Graphite Spin Columns 30 PACK
88303 Pierce TiO2 Phosphopeptide Enrichment Spin Tips 96 EA/PKG
88304 Pierce Detergent Removal Spin Plates 2 PLATES
88305 HiPPR Detergent Removal Spin Column Kit Kit
88306 HiPPR Detergent Removal Spin Columns, 0.1mL Kit
88307 HiPPR Detergent Removal 96-well Spin Plates, 0.1mL Kit
88310 Pierce Kinase Enrichment Kit with ATP Probe Kit
88311 ActivX Desthiobiotin-ATP Probe 16 X 12.6 μg
88312 Pierce Kinase Enrichment Kit with ADP Probe Kit
88313 ActivX Desthiobiotin-ADP Probe 16 X 9.9 μg
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