運(yùn)用高通量熒光定量PCR芯片方法，在一張96孔或384孔板上同時(shí)對某個(gè)信號通路或疾病相關(guān)基因的多個(gè)基因的表達(dá)量變化進(jìn)行檢測，芯片上的基因包括了與研究對象有確定關(guān)系的基因或者待考證的基因；目前啟因生物針對不同的信號通路和疾病相關(guān)基因設(shè)計(jì)了上百種多款功能分類芯片，涵蓋生命科學(xué)研究的各個(gè)領(lǐng)域。
Real-time PCR arrays are tools that allow convenient testing of samples in many assays concurrently, parallel testing of many samples or testing of multiple samples and targets simultaneously.
——Saunders N A（2013）
品牌 | 貨號 | 名稱 | 基因列表 | 價(jià)格 |
啟因生物(wcgene.com) | HUMAN-WC105 | Transcription Factors qpcr array | CBFA2T3, CLOCK, CREBBP (CBP), EGR3, FOXC1, FOXD3, FOXF1, HEY1, HEY2, HEYL, JUN, MSC, MYF5, NOTCH1, PAX6, PBX1, RBPJ, RBPJL, RUNX1 (AML1), RUNX2, SOX9, TCF15. | 300元/樣 |
啟因生物(wcgene.com) | HUMAN-WC108 | Transcription Factors qpcr array | ABL1, BRCA1, BRCA2, CDKN2A, CTNNB1, E2F1, ELK1, ESR1, ETS1, FOS, FOXD3, HIC1, JUN, JUNB, JUND, MDM2, MEN1, MYB, MYC, MYCN, NF1, NFKB1, PML, RARA, RB1, REL, RUNX1, RUNX3,SMAD4, STAT3, TGFB1, TNF, TP53, TP73, TSC1, VHL, WT1, ZHX2. | 300元/樣 |
啟因生物(wcgene.com) | HUMAN-WC129 | Transcription Factors qpcr array | CEBPB, FOSL (FRA-1)1, FOXP3, GATA3, GATA4, HOXA10, HOXA3, ID2, IRF4,IRF8, MAF, NFATC1, NFATC2, NR4A1, NR4A3, POU2F2, REL, RELB, RORA, RORC, RUNX1 (AML1), RUNX3, STAT1, STAT4, STAT6, TOX, ZBTB7B. | 300元/樣 |
啟因生物(wcgene.com) | MOUSE-WC104 | Transcription Factors qpcr array | Cbfa2t3, Clock, Crebbp (Cbp), Egr3, Foxc1, Foxd3,Foxf1, Hey1,Hey2, Heyl, Jun, Msc, Myf5, Notch1, Pax6, Pbx1, Rbpj, Rbpjl, Runx1 (Aml1), Runx2, Sox9, Tcf15. | 300元/樣 |
啟因生物(wcgene.com) | MOUSE-WC107 | Transcription Factors qpcr array | Abl1, Brca1, Brca2, Cdkn2a, Ctnnb1, E2f1, Elk1, Esr1, Ets1, Fos, Foxd3, Hic1, Jun, Junb, Jund, Mdm2, Men1, Myb, Myc, Mycn, Nf1, Nfkb1,Pml, Rara, Rb1, Rel, Runx1, Runx3, Smad4, Stat3, Tgfb1, Tnf, Trp53, Trp73, Tsc1, Vhl, Wt1, Zhx2. | 300元/樣 |
啟因生物(wcgene.com) | MOUSE-WC129 | Transcription Factors qpcr array | Cebpb, Fosl1 (Fra-1), Foxp3, Gata3, Gata4, Hoxa10, Hoxa3, Id2, Irf4, Irf8, Maf, Nfatc1, Nfatc2, Nfatc2ip, Nr4a1, Nr4a3, Pou2f2, Rel,Rora, Rorc, Runx1 (Aml1), Runx3, Stat1, Stat4, Stat6, Zbtb7b, Zeb1. | 300元/樣 |
啟因生物(wcgene.com) | RAT-WC106 | Transcription Factors qpcr array | Cbfa2t3, Clock, Crebbp (Cbp), Egr3, Foxd3, Hey1, Hey2, Heyl, Jun,Myf5, Notch1, Pax6, Pbx1, Rbpjl, Runx1 (Aml1), Runx2, Sox9. | 300元/樣 |
| 啟因生物(wcgene.com) | RAT-WC109 | Transcription Factors qpcr array | Abl1, Brca1, Brca2, Cdkn2a (p16Ink4), Ctnnb1, E2f1, Elk1, Esr1 (ERa), Ets1, Fos, Foxd3,Hic1, Jun, Junb, Jund, Mdm2, Men1, Myc, Mycn, Nf1, Nfkb1, Rara, Rb1, Rel, Runx1 (Aml1),Runx3, Smad4, Stat3, Tgfb1, Tnf, Tp53, Tp73, Tsc1, Vhl, Wt1, Zhx2. | 300元/樣 |
| 啟因生物(wcgene.com) | RAT-WC135 | Transcription Factors qpcr array | Cebpb, Fosl1 (Fra-1), Foxp3, Gata3, Gata4, Hoxa11, Id2, Irf4, Irf8, Maf, Nfatc2,Nfatc2ip, Nr4a1, Nr4a3, Pou2f2, Rel, Rora, Rorc, Runx1 (Aml1), Runx3, Stat4, Stat6, Zbtb7b, Zeb1. | 300元/樣 |
檢測流程： 您只需提供樣品，其他工作交給我們。
1. 樣品RNA提??；
2. RNA質(zhì)量檢測，檢測RNA純度及完整度，提供RNA質(zhì)量報(bào)告；
3. cDNA合成，使用逆轉(zhuǎn)錄酶，將樣品RNA逆轉(zhuǎn)錄為cDNA；
4. 實(shí)時(shí)定量PCR反應(yīng)；
5. 數(shù)據(jù)分析，計(jì)算PCR芯片中的各個(gè)Ct值，采用ΔΔCt方法比較基因的表達(dá)變化，并利用公司自主開發(fā)的數(shù)據(jù)分析軟件進(jìn)行比較。
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