wako 125-05061賴氨酰肽鏈內(nèi)切酶 Lysyl Endopeptidase
【產(chǎn)品詳情】
賴氨酰肽鏈內(nèi)切酶
品牌:WAKO 和光
級別:質(zhì)譜級
wako 125-05061賴氨酰肽鏈內(nèi)切酶 Lysyl Endopeptidase
英文品名:Lysyl Endopeptidase?, Mass Spectrometry Grade
貨號:125-05061
規(guī)格:5×20 μg
應(yīng)用:蛋白質(zhì)組學(xué)。
用途:賴氨酰肽鏈內(nèi)切酶,蛋白測序、質(zhì)譜分析,多肽合成
wako 125-05061賴氨酰肽鏈內(nèi)切酶 Lysyl Endopeptidase
【實(shí)驗(yàn)原理】
賴氨酰肽鏈內(nèi)切酶是質(zhì)譜分析前處理時(shí)常用的蛋白分解酶即賴氨酰肽鏈內(nèi)切酶,賴氨酰肽鏈內(nèi)切酶可以特異性切除賴氨酸基團(tuán)C 末端的多肽,賴氨酰肽鏈內(nèi)切酶可用于蛋白測序分析和Lys-X 化合物的酶合成。
若同時(shí)使用賴氨酰肽鏈內(nèi)切酶和胰酶,可更好地切斷賴氨酸基團(tuán)的多肽,增加多肽的數(shù)量。賴氨酰肽鏈內(nèi)切酶已按照使用習(xí)慣做成小包裝,是方便使用賴氨酰肽鏈內(nèi)切酶的冷凍干燥品。
【產(chǎn)品特點(diǎn)】
賴氨酰肽鏈內(nèi)切酶Lysyl Endopeptidase 125-05061 wako
1)賴氨酰肽鏈內(nèi)切酶用于蛋白質(zhì)譜分析:高特異性、高蛋白質(zhì)消化率;
2)賴氨酰肽鏈內(nèi)切酶能增加肽段數(shù)量、提高裂解效率;
3)賴氨酰肽鏈內(nèi)切酶獨(dú)立包裝,20 μg/一支
【結(jié)果】表明Lep 酶解的錯誤裂解率低。
Tp 酶解時(shí)加入Lep 后,錯誤裂解率有所降低,同時(shí),賴氨酰肽鏈內(nèi)切酶可以鑒定出更多的多肽。
Tp | Lep | Lep +Tp | |
裂解位點(diǎn) | 精氨酸和賴氨酸的C 端 | 賴氨酸的C端 | 精氨酸和賴氨酸的C端 |
錯誤裂解率( 錯誤裂解所占比例) | 多(8 %) | 很少(0 %) | 少(3 %) |
鑒定出的多肽數(shù)量 | 17 | 19 | 22 |
賴氨酰肽鏈內(nèi)切酶Lysyl Endopeptidase 125-05061 wako
Lep+Tp 賴氨酰肽鏈內(nèi)切酶切后,可以在m/z=2000 時(shí)得到吸收峰,而單獨(dú)的Tp 酶切在m/z=2000 時(shí)沒有吸收峰。該結(jié)果表明Lep 可以提高測序覆蓋度。
賴氨酰肽鏈內(nèi)切酶Lysyl Endopeptidase 125-05061 wako
WAKO 其他產(chǎn)品清單:
Wako 125-05061 Lysyl Endopeptidase, Mass Spectrometry Grade 現(xiàn)貨
貨號 品名 規(guī)格 Cas
012-01965 Alminium Oxide 500g -
012-08643 Amino Acids Mixture Standard Solution, Type B 1mL×5A -
013-08391 Amino Acids Mixture Standard Solution, Type H 5mL -
015-14461 Amino Acids Mixture Standard Solution, Type AN-2 5mL -
015-25191 Anti Phosphorylated α-Synuclein, Monoclonal Antibody (pSyn#64) 50ul -
011-14463 Amino Acids Mixture Standard Solution, Type AN-2 1mL×5A -
011-19365 2,2'-Azobis[2-(2-imidazolin-2-yl)propane]
Dihydrochloride 500g 27776-21-2
016-08641 Amino Acids Mixture Standard Solution, Type B 5mL -
Wako 125-05061 Lysyl Endopeptidase, Mass Spectrometry Grade 現(xiàn)貨
016-20001 Anti Iba1, Rabbit (for Western Blotting) 50μg -
016-23281 Anti Rat P2X4, Monoclonal Antibody 50ug -
017-19362 2,2'-Azobis[2-(2-imidazolin-2-yl)propane] Dihydrochloride 25g 27776-21-2
018-08721 Agar, Powder 100g 9002-18-0
018-22021 Anti Mouse Ago2, Monoclonal Antibody 100μL -
019-03435 Ammonium sulfate 500g 7783-20-2
019-19741 Anti Iba1, Rabbit (for Immunocytochemistry) 50μg -
019-22291 Anti Olfactory Marker Protein, Goat 100μL -
021-02195 Boric acid 500g 10043-35-3
Wako 125-05061 Lysyl Endopeptidase, Mass Spectrometry Grade 現(xiàn)貨
021-16201 BES-H2O2(Cell-impermeant) 1mg -
031-17601 Collagenase Type I 100mg 9001-12-1
032-08385 Citric Acid Monohydrate 500g 5949-29-1
032-15372 Casein Phosphopeptide 25g -
034-06363 N-Caprylic Acid 25mL 124-07-2
034-16111 Cefotaxime Sodium Salt 1g 64485-93-4
035-04595 Creatinine 500g 60-27-5
035-17604 Collagenase Type I 1g 9001-12-1
038-23221 α-Casein, from Bovine Milk, Dephosphorylated 1mg -
039-01335 Carboxymethyl Cellulose Sodium Salt 500g 9004-32-4
Wako 125-05061 Lysyl Endopeptidase, Mass Spectrometry Grade 現(xiàn)貨
021-17801 BES-So-AM 1mg 936356-51-3
022-07985 γ-Butyrolactone 500mL 96-48-0
025-03195 Buffer Solution Standard (Phosphate pH Standard Equimolal Solution)
pH6.86 (25 degrees C) 500mL -
047-18863 Dexamethasone 100mg 50-02-2
049-18801 4',6-Diamidino-2-phenylindole Dihydrochloride n-Hydrate 10mg 28718-90-3
058-07681 EasySeparator 1set -
025-03195 Buffer Solution Standard (Phosphate pH Standard Equimolal Solution)
pH6.86 (25 degrees C) 500mL -
Wako 125-05061 Lysyl Endopeptidase, Mass Spectrometry Grade 現(xiàn)貨
019-08393 Amino Acids Mixture Standard Solution, Type H 5Ax1mL -
019-11941 Active Carbon-impregnated Silica Gel 10g -
019-19741 Anti Iba1, Rabbit (for Immunocytochemistry) 50μg -
025-15481 BES-Thio 1mg -
026-16371 BF-170 1mg -
027-01276 Benzyl Alcohol 500mL 100-51-6
028-03185 Buffer Solution Standard (Phthalate pH Standard Solution)
pH4.01 (25 degrees C) 500mL -
028-03185 Buffer Solution Standard (Phthalate pH Standard Solution)
Wako 125-05061 Lysyl Endopeptidase, Mass Spectrometry Grade 現(xiàn)貨
pH4.01 (25 degrees C) 500mL -
028-03205 Buffer Solution Standard (Tetraborate pH Standard Solution)
pH9.18 (25 degrees C) 500mL -
077-03155 Gelatin 500g 9000-70-8
081-00136 Heparin Sodium 10mu 9041-08-1
088-00705 L-Histidine Hydrochloride Monohydrate 500g 5934-29-2
093-06471 Insulin, Human, recombinant 50mg -
097-06474 Insulin, Human, recombinant 1g 11061-68-0
120-04891 L-012 100mg -
125-05061 Lysyl Endopeptidase, Mass Spectrometry Grade 5x20μg -
Wako 125-05061 Lysyl Endopeptidase, Mass Spectrometry Grade 現(xiàn)貨
129-02541 Lysyl Endopeptidase( R) 10AU - -
129-04861 LY 294002 5mg 154447-36-6
130-07871 Melibiose 5g 585-99-9
028-16211 BES-So (Cell-impermeant) 1mg -
028-17811 BES-H2O2-Ac 1mg -
029-16361 BF-168 1mg -
039-23511 CultureSure? Freezing Medium 100ml -
041-18861 Dexamethasine 1g 50-02-2
042-29445 Dibasic Sodium Phosphate Hydrate 500g 10039-32-4
043-22611 Dextran 200,000 100g 9004-54-0
064-05381 Fibroblast Growth Factor (basic)(FGFb / bFGF / FGF2),
Wako 125-05061 Lysyl Endopeptidase, Mass Spectrometry Grade 現(xiàn)貨
Human, recombinant, Animal-derived-free 50ug 106096-93-9
071-02293 gamma-Globulin, from Human Serum 5g 9007-83-4
075-03075 Gellan Gum 500g 71010-52-1
131-00405 Magnesium Sulfate 500g 10034-, 99-8
136-03032 Methyl Red 25g 493-52-7
137-06085 Molecular Sieves 4A1/16 500g 70955-01-0
138-07872 Melibiose 25g 585-99-9
145-08221 Nuclease P1 500u 54576-84-0
145-08221 Nuclease P1 500u 54576-84-0
162-09115 Polyethylene Glycol 4,000 500g 25322-68-3
Wako 125-05061 Lysyl Endopeptidase, Mass Spectrometry Grade 現(xiàn)貨
163-03545 Potassium Chloride 500g 7447-40-7
164-21655 N/400 Potassium Polyvinyl Sulfate Solu 500mL 26837-42-3
191-01785 Trisodium Citrate Dihydrate 500g 6132-04-3
193-02041 Glycerol 2-Phosphate Disodium Salt n-Hydrate 100g 819-83-0
195-12854 Streptavidin, from Streptomyces avidinii 5mg 9013-20-1
195-16031 StemSure (R) Freezing Medium 100ml -
196-00015 Sucrose 500g 57-50-1
Wako 125-05061 Lysyl Endopeptidase, Mass Spectrometry Grade 現(xiàn)貨
196-08675 Sodium Dodecyl Sulfate 500g 151-21-3
197-07485 Sodium Sulfate 500g 7757-82-6
199-02825 Sodium Dihydrogenphosphate Dihydrate 500g 13472-35-0
203-17561 Trichostatin A 1mg 58880-19-6
207-00055 L(+)-Tartaric Acid 500g 87-69-4
208-08385 1mol/l Triethylammonium Hydrogencarbonate Solution 500mL 15715-58-9
223-02112 VA-044 25g 27776-21-2
225-02111 VA-044 100g 27776-21-2
165-09105 Polyethylene Glycol 2,000 500g 25322-68-3
165-17035 Polyvinylpyrrolidone K 30 500g 9003-39-8
167-09045 Polyethylene Glycol 200 500g 25322-68-3
169-09125 Polyethylene Glycol 6,000 500g 25322-68-3
Wako 125-05061 Lysyl Endopeptidase, Mass Spectrometry Grade 現(xiàn)貨
185-01003 Rifampicin 5g 13292-46-1
186-01114 all-trans-Retinoic Acid 50mg 302-79-4
189-01001 Rifampicin 1g 13292-46-1
190-05535 Trisodium Citrate Dihydrate 500g 6132-04-3
191-01665 Sodium Chloride 500g 7647-14-5
227-01071 Valproic Acid 5g 99-66-1
231-02011 Wetting Tension Test Mixture No.52.0 50mL -
232-01941 Wetting Tension Test Mixture No.42.0 50mL -
233-01971 Wetting Tension Test Mixture No.45.0 50mL -
Wako 125-05061 Lysyl Endopeptidase, Mass Spectrometry Grade 現(xiàn)貨
234-02001 Wetting Tension Test Mixture No.50.0 50mL -
235-01931 Wetting Tension Test Mixture No.41.0 50mL -
235-02031 Wetting Tension Test Mixture No.56.0 50mL -
236-01961 Wetting Tension Test Mixture No.44.0 50mL -
238-01921 Wetting Tension Test Mixture No.40.0 50mL -
238-02021 Wetting Tension Test Mixture No.54.0 50mL -
238-02141 Wetting Tension Test Mixture No.73.0 50mL 7732-18-5
253-00513 Y-27632 5mg 331752-47-7
257-00511 Y-27632 1mg 331752-47-7
Wako 125-05061 Lysyl Endopeptidase, Mass Spectrometry Grade 現(xiàn)貨
290-34251 Presep-C DNPH 20EA -
296-63801 LabAssay? Phospholipid 1300tests -
298-64101 Fructooligosaccharides standard set set -
302-93561 Phos-tag (TM) Agarose 0.5mL -
304-93521 Phos-tag (TM) Acrylamide AAL-107 10mg -
304-93521 Phos-tag (TM) Acrylamide AAL-107 10mg -
290-34251 Presep-C DNPH 20EA -
290-63701 LabAssay? Triglyceride 1000tests -2016-2-29
290-65901 LabAssay? Creatinine 500tests -
291-58601 Lab Assay ALP 900tests -
293-51601 Endotoxin Extracting Solution for LAL Test 10mL x 4 -
294-62001 DsDD cDNA Subtraction kit Wako 5tests -
294-63601 LabAssay? NEFA 750tests -
294-65801 LabAssay? Cholesterol 1000tests -
295-50201 DNA Extractor? Kit 50tests -
295-51301 Limulus ES-II Single Test Wako 25 tests -
295-51301 Limulus ES-II Single Test Wako 25 tests -
295-73401 Fructooligosaccharides Set Set -
304-93526 Phos-tag (TM) Acrylamide AAL-107 5mM Aqueous Solution 0.3ml -
305-93551 Phos-tag? Mass Analytical Kit set -
308-93541 Phos-tag (TM) Biotin BTL-105 10mg -
308-93563 Phos-tag (TM) Agarose 3mL -
308-97201 Phos-tag Biotin BTL-1111 1mM Aqueous Solution 0.1000mL
325-88521 Lipase PS Amano SD 10g -
298-65701 LabAssay? Glucose 1000tests -
300-93523 Phos-tag (TM) Acrylamide AAL-107 2mg -
301-93531 Phos-tag (TM) Biotin BTL-104 10mg -
301-99413 Phos-tagTM Biotin BTL-104 1mM Aqueous Solution[for Campaign] 0.1mL 753451-66-0
302-93522 Phos-tagTM Acrylamide AAL-107 5mM Aqueous Solution[for Campaign] 0.3mL 871839-54-2
631-00651 Cellmatrix Type I -A (Collagen, Type I, 3 mg/mL, pH 3.0) 20mL -
637-00653 Cellmatrix Type I -A (Collagen, Type I, 3 mg/mL, pH 3.0) 100mL -
637-00653 Cellmatrix Type I -A (Collagen, Type I, 3 mg/mL, pH 3.0) 100mL -
638-01021 Endotoxin Standad /JPSE 10000un -
986-10001 Anti asialo GM1 (Rabbit), (014-09801) 1mL 617-45-8
圖片僅供參考,請以實(shí)物為準(zhǔn)。
我司所銷售的化學(xué)試劑、原料等所有產(chǎn)品(包括但不限于抗生素類、蛋白質(zhì)類、試劑盒類產(chǎn)品等)*用于科學(xué)研究用途,不得作用于人體。