小鼠可溶性E選擇素(sE-selectin)ELISA 檢測(cè)試劑盒,小鼠可溶性E選擇素(sE-selectin)ELISA 檢測(cè)試劑盒
小鼠可溶性E選擇素(sE-selectin)ELISA 檢測(cè)試劑盒*試驗(yàn)原理:
ZR試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)(ELISA).已知ZR濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測(cè)。先將ZR和生物素標(biāo)記的抗體同時(shí)溫育。洗滌后,加入親和素標(biāo)記過的HRP。再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時(shí)作用。產(chǎn)生顏色。顏色的深淺和樣品中ZR的濃度呈比例關(guān)系。
試劑盒內(nèi)容及其配制
試劑盒成份(2-8℃保存)
96孔配置
48孔配置
配制
96/48人份酶標(biāo)板
1塊板(96T)
半塊板(48T)
即用型
塑料膜板蓋
1塊
半塊
即用型
標(biāo)準(zhǔn)品:40nmol/L
1瓶(0.6ml)
1瓶(0.3ml)
按說明書進(jìn)行稀稀
空白對(duì)照
1瓶(1.0ml)
1瓶(0.5ml)
即用型
標(biāo)準(zhǔn)品稀釋緩沖液
1瓶(5ml)
1瓶(2.5ml)
即用型
生物素標(biāo)記的抗ZR抗體
1瓶(6ml)
1瓶(3.0ml)
即用型
親和鏈酶素-HRP
1瓶(10ml)
1瓶(5.0ml)
即用型
洗滌緩沖液
1瓶(20ml)
1瓶(10ml)
按說明書進(jìn)行稀釋
底物A
1瓶(6.0ml)
1瓶(3.0ml)
即用型
底物B
1瓶(6.0ml)
1瓶(3.0ml)
即用型
終止液
1瓶(6.0ml)
1瓶(3.0ml)
即用型
標(biāo)本稀釋液
1瓶(12ml)
1瓶(6.0ml)
即用型
【簡單介紹】
小鼠可溶性E選擇素(sE-selectin)ELISA試劑盒"標(biāo)本取樣的基本要求 :
*標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn)。若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融
*不能檢測(cè)含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
RS12468B 兔子可溶性粘附分子(Sam)elisa試劑盒 Rabbit soluble adhesion molecules,Sam Elisa試劑盒
RS12469B 兔子抗中性粒細(xì)胞顆??贵w(ANGA)elisa試劑盒 Rabbit anti-neutrophil granules antibody,ANGA Elisa試劑盒
RS12470B 兔子孕激素/孕酮(PROG)elisa試劑盒 Rabbit Progesterone,PROG Elisa試劑盒
RS12471B 兔子膠原酶II(Collagenase II)elisa試劑盒 Rabbit Collagenase Type II Elisa試劑盒
RS12472B 兔子膠原酶I(Collagenase I)elisa試劑盒 Rabbit Collagenase I Elisa試劑盒
RS12473B 兔子腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體3(TRAIL-R3)elisa試劑盒 Rabbit tumor necrosis factor-related apoptosis-inducing ligand 3,TRAIL-R3 Elisa試劑盒
RS12474B 兔子巨噬細(xì)胞炎性蛋白1α(MIP-1α/CCL3)elisa試劑盒 Rabbit Macrophage Inflammatory Protein-1α,MIP-1α Elisa試劑盒
RS12475B 兔子神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)elisa試劑盒 Rabbit glial fibrillary acidic protein,GFAP Elisa試劑盒
RS12476B 豚鼠白介素4(IL-4)elisa試劑盒 Guinea pig interleukin 4,IL-4 Elisa試劑盒
RS12477B 豚鼠免疫球蛋白G(IgG)elisa試劑盒 Guinea pig Immunoglobulin G,IgG Elisa試劑盒
RS12478B 豚鼠血清總補(bǔ)體(CH50)elisa試劑盒 Guinea Pig 50% complement hemolysis,CH50 Elisa試劑盒
RS12479B 豚鼠組胺(HIS)elisa試劑盒 guinea pig Histamine,HIS Elisa試劑盒
RS12480B 豚鼠轉(zhuǎn)化生長因子β1(TGF-β1)elisa試劑盒 guinea pig transforming growth factor β1,TGF-β1 Elisa試劑盒
RS12481B 豚鼠腫瘤壞死因子α(TNF-α)elisa試劑盒 Guinea pig Tumor necrosis factor α,TNF-α Elisa試劑盒
RS12482B 豚鼠生長激素(GH)elisa試劑盒 Guinea pig Growth hormone,GH Elisa試劑盒
RS12483B 豚鼠內(nèi)皮素1(ET-1)elisa試劑盒 guinea pig Endothelin 1,ET-1 Elisa試劑盒
RS12484B 豚鼠免疫球蛋白E(IgE)elisa試劑盒 guinea pig Immunoglobulin E,IgE Elisa試劑盒
RS12485B 豚鼠免疫球蛋白A(IgA)elisa試劑盒 guinea pig Immunoglobulin A,IgA Elisa試劑盒
RS12486B 豚鼠基質(zhì)金屬蛋白酶抑制因子1(TIMP-1)elisa試劑盒 guinea pig tissue inhibitors of metalloproteinase 1,TIMP-1 Elisa試劑盒
RS12487B 豚鼠基質(zhì)金屬蛋白酶9(MMP-9)elisa試劑盒 guinea pig matrix metalloproteinase 9,MMP-9 Elisa試劑盒
RS12488B 豚鼠肝細(xì)胞生長因子(HGF)elisa試劑盒 guinea pig hepatocyte growth factor,HGF Elisa試劑盒 ab13941 Mouse monoclonal [1691 ] to Chlamydia Pneumoniae 50ug 3450
ab13942 Goat polyclonal to Chlamydia trachomatis MOMP 1ml 3450
ab13943 Rabbit polyclonal to Chlamydia trachomatis 1ml 3450
ab13945 Rabbit polyclonal to GPCR GPR87 50ug 3450
ab13948 Rabbit polyclonal to SOCS4 100ug 3450
ab13949 Rabbit polyclonal to SOCS5 100ug 3450
ab13950 Rabbit polyclonal to SOCS6 100ug 3450
ab13951 Rabbit polyclonal to SOCS7 100ug 3450
ab13952 Rabbit polyclonal to CISH 100ug 3450
ab13953 Rabbit polyclonal to Lck 100ug 3450
ab13954 Rabbit polyclonal to Yes1 100ug 3450
ab13955 Rabbit polyclonal to Fyn 100ug 3450
ab13956 Rabbit polyclonal to Syk 100ug 3450
ab13957 Rabbit polyclonal to ZAP70 100ug 3450
ab13958 Rabbit polyclonal to Lyn 100ug 3450
ab13959 Rabbit polyclonal to Sortase 100ug 3450
ab1396 Rabbit polyclonal to GAL4 - ChIP Grade 50ug 3680
ab13960 Rabbit polyclonal to ESAT6 100ug 3450
ab13961 Rabbit polyclonal to Cable pilus 100ug 3450
ab13962 Rabbit polyclonal to EEF1D 100ug 2768
ab13963 Rabbit polyclonal to CD33 100ug 3450
ab13964 Rabbit polyclonal to Siglec 7 100ug 3450
ab13967 Mouse monoclonal [vpg 50] to FIV p24 1ml 3450
ab13968 Rabbit polyclonal to Plasminogen 100ug 3450
ab13969 Mouse monoclonal [vpg 68] to FIV p120 1ml 3450
ab13970 Chicken polyclonal to GFP 100ul 3738
ab13971 Mouse monoclonal [B007] to HSV1 + HSV2 500ug 3450
ab13972 Mouse monoclonal [6G3] to liver Arginase 50ug 3450
ab13973 Rabbit polyclonal to CCR1 50ug 3450
ab13974 Rabbit polyclonal to CCR2 50ug 3450
ab13975 Mouse monoclonal [3G6D9] to Cardiotrophin 1 50ug 3450
ab13976 Rabbit polyclonal to ADAMTS5 50ug 3623
ab13977 Rabbit polyclonal to CCR3 50ug 3450
ab13978 Rabbit polyclonal to Uteroglobin 50ug 3450
zui重要是安全性
1. 避免直接接觸終止液和底物A、B。一旦接觸到這些液體,請(qǐng)盡快用水沖洗。
2. 實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。
3. 不要用嘴吸取試劑盒里的任何成份。
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