LIF, mouse, recombinant
LIF (leukemia inhibitory factor, myeloid leukemia inhibitory factor) a pleiotropic lymphoid factor was initially described as a factor that inhibits the proliferation of myeloid leukemia cells and induces their differentiation into macrophages. Other activities of LIF include inhibition of adipogenesis, cholinergic neuron differentiation and bone metabolism. Human and mouse LIF share 78% homology and activities of LIF are not species-specific. Human LIF is active on mouse cells, but murine LIF is not active on human cells.
 
Lot Number
 
01-1-114-7132
 
Available Sizes
 
10µg, 50µg, 100µg, 1mg
 
Source
 
ISOkine™ recombinant mouse LIF is produced in the endosperm tissue of barley grain (Hordeum vulgare).
 
Formulation
 
PBS, pH 7.2, sterile filtered.
 
Purity
 
Greater than 95% by SDS-PAGE gel analysis
 
Reconstitution
 
Note: Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 µg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
 
Stability
 
The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediay or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
 
Biological Activity
 
Each batch of ISOkine™ growth factor is tested for bioactivity and verified to have comparable activity to a commercial source. The bioactivity of ISOkine™ recombinant murine LIF was determined by its ability to induce differentiation of murine M1 myeloid leukemia cells where 50 U is defined as the amount of mLIF required to induce differentiation of 50% of M1 cells. 10 ug of ISOkine™ mLIF protein is equivalent to approximay 10e6 activity units. The ISOkine™ mLIF has been validated in standard mESC based gene targeting for generation of knock-out mice. We recommend that the optimal concentration for each specific application be determined by an initial dose-response assay.
 
Endotoxin Level
 
Endotoxin level is less than 0.005ng per µg of ISOkine™ product (< 0.05EU/µg).
 
MAT assay
 
Purified ISOkine™ product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha and IL-1beta induction. Ref. The Blood Bank, University Hospital of Iceland, Reykjavik, Iceland
 
Molecular Weight
 
Recombinant mouse LIF contains 188 amino acids and a 10 a.a. histidine-based tag for a total length of 199 a.a. and has a predicted molecular mass of 21.2 kDa. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 28-30 kDa in SDS-PAGE.
DisclaimerCertain biotechnological methods involving the use of this product may be protected by third party patents, such as but not limited to culturing and maintaining embryonic stem cell in vitro in cell culture for commercial purposes. Commercial use of such methods may require licenses in certain countries. Purchase of this product does not include a license to perform patented applications.

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