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H2DCFDA試劑返回列表頁(yè)

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型號(hào)
HY-D0940
品牌
MCE
廠商性質(zhì)
代理商
所在地
杭州市

規(guī)格

10 mM * 1 mL in DMSO 550元 10000支可售

10 mg 250元 10000支可售

25 mg 370元 10000支可售

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更新時(shí)間：2023-07-01 10:40:36瀏覽次數(shù)：745次

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產(chǎn)地	進(jìn)口	級(jí)別	其他

H2DCFDA試劑

H2DCFDA (DCFH-DA) 是一種可滲透細(xì)胞的，用于檢測(cè)細(xì)胞內(nèi)活性氧 (ROS) 的探針 (Ex/Em=488/525 nm)。

H2DCFDA試劑生物活性

H2DCFDA (DCFH-DA) is a cell-permeable probe used to detect intracellular reactive oxygen species (ROS) (Ex/Em=488/525 nm)

分子量

487.29

性狀

Solid

Formula

C₂₄H₁₆Cl₂O₇

CAS 號(hào)

4091-99-0

Emission (Em)

517

Excitation (Ex)

492

運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.

儲(chǔ)存方式

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性數(shù)據(jù)

In Vitro:

DMSO : 125 mg/mL (256.52 mM; Need ultrasonic)

Ethanol : 20 mg/mL (41.04 mM; Need ultrasonic)

配制儲(chǔ)備液

濃度溶劑體積質(zhì)量	1 mg	5 mg	10 mg

1 mM	2.0522 mL	10.2608 mL	20.5217 mL
5 mM	0.4104 mL	2.0522 mL	4.1043 mL
10 mM	0.2052 mL	1.0261 mL	2.0522 mL

請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲(chǔ)備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 儲(chǔ)存時(shí)，請(qǐng)?jiān)?6 個(gè)月內(nèi)使用，-20°C 儲(chǔ)存時(shí)，請(qǐng)?jiān)?1 個(gè)月內(nèi)使用。

In Vivo:

請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥方式選擇適當(dāng)?shù)娜芙夥桨?。以下溶解方案都?qǐng)先按照 In Vitro 方式配制澄清的儲(chǔ)備液，再依次添加助溶劑：

——為保證實(shí)驗(yàn)結(jié)果的可靠性，澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用；以下溶劑前顯示的百
分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

1.

請(qǐng)依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: 2.08 mg/mL (4.27 mM); Suspended solution; Need ultrasonic
2.

請(qǐng)依序添加每種溶劑： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.08 mg/mL (4.27 mM); Clear solution
3.

請(qǐng)依序添加每種溶劑： 10% DMSO 90% corn oil
Solubility: ≥ 2.08 mg/mL (4.27 mM); Clear solution

染色示例	Description: H2DCFDA (DCFH-DA) is a ROS production probe with green fluorescence. Method: For cell staining. 1. Stain cells with H2DCFDA. 2. Capture staining cells by spinning-disk confocal microscopy (every 5 min for 6 h). Description: H2DCFDA (DCFH-DA) is a ROS production probe with green fluorescence. Method: For cell staining. 1. Remove the cell culture medium and replaced by fresh medium containing H2DCFDA and incubated with cultured cells for 30 min. 2. Wash cells with PBS for three times. 3. Use a confocal laser scanning microscopy (FV1000, Olympus Company, Japan) to detect the intracellular ROS generation. Description: H2DCFDA (DCFH-DA) is a ROS production probe with green fluorescence. Method: For cell staining. 1. Testing cells are seeded into a 6-well microplate at a density of 10⁵/well and cultured overnight at 37°C in 5% CO₂. Fresh medium with 50 μg/mL of different materials is added to each well and cultured for 16 h. 2. Wash cells with PBS and stain cells with H2DCFDA (20 μM; 1 h; 37°C; dark). 3. Cells are trypsinized, washed, resuspended in PBS (0.2 mL), analyzed by a flow cytometer and use a confocal laser scanning microscopy (FV1000, Olympus, Tokyo, Japan) for image. Description: H2DCFDA (DCFH-DA) is a ROS production probe with green fluorescence. Method: For cell staining. 1. Wash cultured cells with PBS for three times. 2. Treat testing cells with H2DCFDA in culture medium for 0.5 h in a cell incubator. 3. Use a confocal laser scanning microscopy (Olympus, FV3000) for image. Cells are analyzed on a BD LSRI Fortessa flow cytometer analyzer to quantify results. Description: H2DCFDA (DCFH-DA) is a ROS production probe with green fluorescence. Method: For cell staining.

染色示例

Description: H2DCFDA (DCFH-DA) is a ROS production probe with green fluorescence.
Method: For cell staining.
1. Stain cells with H2DCFDA.
2. Capture staining cells by spinning-disk confocal microscopy (every 5 min for 6 h).
Description: H2DCFDA (DCFH-DA) is a ROS production probe with green fluorescence.
Method: For cell staining.
1. Remove the cell culture medium and replaced by fresh medium containing H2DCFDA and incubated with cultured cells for 30 min.
2. Wash cells with PBS for three times.
3. Use a confocal laser scanning microscopy (FV1000, Olympus Company, Japan) to detect the intracellular ROS generation.
Description: H2DCFDA (DCFH-DA) is a ROS production probe with green fluorescence.
Method: For cell staining.
1. Testing cells are seeded into a 6-well microplate at a density of 10⁵/well and cultured overnight at 37°C in 5% CO₂. Fresh medium with 50 μg/mL of different materials is added to each well and cultured for 16 h.
2. Wash cells with PBS and stain cells with H2DCFDA (20 μM; 1 h; 37°C; dark).
3. Cells are trypsinized, washed, resuspended in PBS (0.2 mL), analyzed by a flow cytometer and use a confocal laser scanning microscopy (FV1000, Olympus, Tokyo, Japan) for image.
Description: H2DCFDA (DCFH-DA) is a ROS production probe with green fluorescence.
Method: For cell staining.
1. Wash cultured cells with PBS for three times.
2. Treat testing cells with H2DCFDA in culture medium for 0.5 h in a cell incubator.
3. Use a confocal laser scanning microscopy (Olympus, FV3000) for image. Cells are analyzed on a BD LSRI Fortessa flow cytometer analyzer to quantify results.
Description: H2DCFDA (DCFH-DA) is a ROS production probe with green fluorescence.
Method: For cell staining.